马来西亚无刺蜂乙醇蜂花粉提取物对MCF-7和MCF-10A细胞株的治疗评价

IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY

Pertanika Journal of Tropical Agricultural Science Pub Date : 2022-12-22 DOI:10.47836/pjtas.46.1.03

N. Fadzilah, W. W. Wan Omar

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引用次数: 0

摘要

蜂花粉是一种营养全面、具有消除自由基的治疗作用的完整食品。采用马来西亚原生的三种无刺蜜蜂:Geniotrigona胸蜂、Heterotrigona itama蜂和Tetrigona apicalis蜂花粉乙醇提取物(BPE)制备蜂花粉乙醇提取物。本研究使用的方法是台盼蓝排除法测定培养的乳腺腺癌人细胞系(MCF-7)和乳腺上皮人细胞系(MCF-10A)的细胞增殖活性。此外，还分析了治疗指数(TI)，以评估BPE对肿瘤细胞和正常细胞的相对抗增殖活性。胸椎外三角肌BPE的治疗指数(TI = 3.12)高于itama h (TI = 1.16)和T. apicalis (TI = 0.90)。由于不同的花粉采集活动，每个物种代表不同的生物活性化合物。因此，TI最高的种(胸胸草属)可能是开发潜在化疗药物的潜在候选人。

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Therapeutic Evaluation of Ethanolic Bee Pollen Extract from Malaysian Stingless Bee in MCF-7 and MCF-10A Cell Lines

Bee pollen is a complete food containing comprehensive nutrients and therapeutic properties that could eliminate free radicals. Three stingless bee species native to Malaysia were used to prepare bee pollen ethanolic extracts (BPE): Geniotrigona thoracica, Heterotrigona itama, and Tetrigona apicalis. The methodology used in this study was a trypan blue exclusion assay for cell proliferation activity in cultured breast adenocarcinoma human cell lines (MCF-7) and mammary epithelial human cell lines (MCF-10A). In addition, the therapeutic index (TI) was analyzed to assess the relative antiproliferative activity of BPE on cancer versus normal cells. Geniotrigona thoracica, BPE exhibited the highest therapeutic index (TI = 3.12) compared to H. itama (TI = 1.16) and T. apicalis (TI = 0.90) BPE. Each species represents different bioactive compounds due to different pollen foraging activities. Therefore, the highest TI species (G. thoracica) could be a potential candidate to be developed as a potential chemotherapeutic agent.

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来源期刊

Pertanika Journal of Tropical Agricultural Science Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.10

自引率

16.70%

发文量