从葡萄糖和木糖中脂质积累的工程，天然产油酿酒酵母菌株

IF 14.4 Q1 ENERGY & FUELS

Biofuel Research Journal-BRJ Pub Date : 2018-06-01 DOI:10.18331/BRJ2018.5.2.3

E. Knoshaug, S. V. Wychen, Arjun Singh, Min Zhang

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引用次数: 13

摘要

酿酒酵母是一种众所周知的用于酒精发酵的工业酵母，历史上并不知道它会积累脂质。筛选了工业应用中使用的四种酿酒酵母菌株，以检测其积累中性脂的能力。只有一种，D5A，被发现积累高达20%的干细胞重量(dcw)脂质。该菌株通过敲除adp激活的丝氨酸/苏氨酸激酶(SNF1)进一步工程化，使脂质积累增加到35%。此外，我们设计D5A利用木糖，发现D5A从木糖作为唯一的碳源积累高达37%的dcw脂质。此外，我们过表达不同的二酰基甘油酰基转移酶(DGA1)基因，并将脂质积累提高到50%。脂肪酸形态分析表明，94%的提取脂质由C16:0(棕榈酸)、C16:1n7(棕榈油酸)、C18:0(硬脂酸)、C18:1n7(异丙酸)和C18:1n9(油酸)5种脂肪酸组成，其相对分布随生长条件的不同而变化。此外，该菌株在生产乙醇的同时也积累了脂质。

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Lipid accumulation from glucose and xylose in an engineered, naturally oleaginous strain of Saccharomyces cerevisiae

Saccharomyces cerevisiae, a well-known industrial yeast for alcoholic fermentation, is not historically known to accumulate lipids. Four S. cerevisiae strains used in industrial applications were screened for their ability to accumulate neutral lipids. Only one, D5A, was found to accumulate up to 20% dry cell weight (dcw) lipids. This strain was further engineered by knocking out ADP-activated serine/threonine kinase (SNF1) which increased lipid accumulation to 35% dcw lipids. In addition, we engineered D5A to utilize xylose and found that D5A accumulates up to 37% dcw lipids from xylose as the sole carbon source. Further we over-expressed different diacylglycerol acyltransferase (DGA1) genes and boosted lipid accumulation to 50%. Fatty acid speciation showed that 94% of the extracted lipids consisted of 5 fatty acid species, C16:0 (palmitic), C16:1n7 (palmitoleic), C18:0 (stearic), C18:1n7 (vaccenic), and C18:1n9 (oleic), while the relative distributions changed depending on growth conditions. In addition, this strain accumulated lipids concurrently with ethanol production.

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来源期刊

Biofuel Research Journal-BRJ ENERGY & FUELS-

CiteScore

22.10

自引率

1.50%

发文量

审稿时长

8 weeks

期刊介绍： Biofuel Research Journal (BRJ) is a leading, peer-reviewed academic journal that focuses on high-quality research in the field of biofuels, bioproducts, and biomass-derived materials and technologies. The journal's primary goal is to contribute to the advancement of knowledge and understanding in the areas of sustainable energy solutions, environmental protection, and the circular economy. BRJ accepts various types of articles, including original research papers, review papers, case studies, short communications, and hypotheses. The specific areas covered by the journal include Biofuels and Bioproducts, Biomass Valorization, Biomass-Derived Materials for Energy and Storage Systems, Techno-Economic and Environmental Assessments, Climate Change and Sustainability, and Biofuels and Bioproducts in Circular Economy, among others. BRJ actively encourages interdisciplinary collaborations among researchers, engineers, scientists, policymakers, and industry experts to facilitate the adoption of sustainable energy solutions and promote a greener future. The journal maintains rigorous standards of peer review and editorial integrity to ensure that only impactful and high-quality research is published. Currently, BRJ is indexed by several prominent databases such as Web of Science, CAS Databases, Directory of Open Access Journals, Scimago Journal Rank, Scopus, Google Scholar, Elektronische Zeitschriftenbibliothek EZB, et al.