森菌汁对链脲佐菌素诱导的糖尿病大鼠胰腺和肝脏组织学变化的影响

Q4 Medicine
Hassan Bilal Ka`bange, Dennis Russa
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引用次数: 0

摘要

背景:木槿和辣木对高血糖动物具有抗糖尿病作用。然而,目前还没有关于其在糖尿病动物中的组织学和生理学变化的报道。目的:观察链脲佐菌素诱导的糖尿病大鼠灌胃莫里泽拉汁后胰腺和肝脏的组织学变化。方法:本研究采用完全随机区组设计(CRBD),选择40只成年雄性Wistar大鼠,9至10周,体重190g–250g进行研究。将动物分为四个主要组(A、B、C和D),每组进一步细分为两组,每组5只大鼠。实验分为急性和亚急性试验,每组10只动物。在实验结束时,处死动物,采集胰腺和肝组织并进行组织学处理。使用光学显微镜(Leica®DM 750)和内置相机(Icc50 HD-47142065)对切片进行检查和拍照,并在40个高功率场中测量Islet细胞的数量。结果:胰腺显微结构显示,在50mg/kg和100mg/kg体重的剂量下,莫里泽拉治疗组的郎格汉斯岛数量显著增加。与两个对照组相比,两个治疗组的肝脏微观结构均呈现正常的组织学结构。此外,Morizella治疗组的肝脏结构恢复正常。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of Pancreatic and Hepatic Histological Changes in Streptozotocin Induced Diabetic Rats Fed with Morizella Juice
Background: Hibiscus sabdariffa and Moringa oleifera has been reported to have ant diabetic effect on hyperglycemic animals. However, there are no reports on its histological and physiological changes in diabetic animals. Objective: To assess the pancreatic and hepatic histological changes in streptozotocin-induced diabetic rats fed with Morizella juice. Methods: A Completely Randomized Block Design (CRBD) was used for this study, 40 adult male Wistar rats, 9 to 10 weeks with a bodyweight 190g – 250g were selected for the study. The animals were divided into four main groups (A, B, C, and D), each group was further subdivided into two groups with 5 rats. The experiments were divided into acute and sub-acute tests of 10 animals per group. At the end of the experiment, animals were sacrificed, pancreatic and liver tissues were harvested and histologically processed. Sections were examined and photographed using a light microscope (Leica® DM 750) with an in-built camera ((Icc50 HD-47142065) and the number of Islet cells was measured in 40 high-power fields. Results: Microscopic structures of the pancreas revealed that the number of Islets of Langerhans was significantly higher in the Morizella treated groups at the dosages of 50mg/kg and 100 mg/kg body weight. The microscopic structure of the liver in both treated groups presented with normal histological architecture when compared with both control groups Conclusion: There was a dose-dependent increase of islets of Langerhans cells in the acute and sub-acute group treated with Morizella extracts compared to the control group. Additionally, the liver architecture was reversed back to the normal in Morizella-treated group.
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来源期刊
Tanzania Journal of Health Research
Tanzania Journal of Health Research Medicine-Medicine (all)
CiteScore
0.20
自引率
0.00%
发文量
20
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