利用下一代测序技术开发和鉴定复活节岛巴斯库Panulirus(十足目:Palinuridae)前16个微卫星位点

Pub Date : 2017-08-01 DOI:10.4067/S0718-19572017000200018

E. Díaz-Cabrera, E. Meerhoff, N. Rojas-Hernández, C. Vega‐Retter, D. Véliz

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引用次数: 2

摘要

由于其文化和经济的重要性，多刺龙虾在复活节岛的特有物种中脱颖而出。18个个体共检测到16个微卫星位点，其中9个为多态性位点。平均每个位点的等位基因数为3.44(2-6)，杂合度为0.11 ~ 0.93。没有一个位点表现出明显的连锁不平衡或偏离HWE。这些新的微型卫星将用于获取关于巴氏矮种的迁移、种群结构和遗传多样性的信息，以改进未来的可持续管理和保护计划。

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Development and characterization of the first 16 microsatellites loci for Panulirus pascuensis (Decapoda: Palinuridae) from Easter Island using Next Generation Sequencing

The spiny lobster Panulirus pascuensis stands out among the endemic species of Easter Island, due to its cultural and economic importance. A total of 16 microsatellite loci were characterized in 18 individuals, 9 of which were polymorphic. The mean number of alleles per locus was 3.44 (2-6) and the observed heterozygosity ranged from 0.11 to 0.93. None of the loci exhibited significant linkage disequilibrium or departures from HWE. These new microsatellites will be used to obtain information about migration, population structure and genetic diversity of P. pascuensis in order to improve the future sustainable management and conservation plans.

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