不同染色载体对血迹mrna谱的影响

Q3 Social Sciences

Journal of Forensic Science and Medicine Pub Date : 2022-04-01 DOI:10.4103/jfsm.jfsm_36_21

Hemiao Zhao, Qingluan Lin, Qi Zhang, Jing Chen, Z. Tu, Ruiqin Yang, Lan Hu, Chong Wang

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引用次数: 0

摘要

与DNA图谱不同，信使核糖核酸图谱在很大程度上受到外部因素的影响。为了分析不同染色载体对血迹信使核糖核酸标记物检测能力的影响，本研究检测了10种载体，包括刀、棉签、纸、塑料、皮革、水泥、筷子、衣服、陶瓷块和墙壁。在外周血样本中检测到五种特异性信使核糖核酸标记物（HBA、HBB、ALAS2、GYPA和SPTB）和管家基因B2M后，未发现携带者的效果存在统计学显著差异。结果表明，在对血迹进行信使核糖核酸检测时，染色载体的作用几乎没有影响。

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The impact of different stain carriers on the mrna profiling from bloodstains

Unlike DNA profiling, mRNA profiling is greatly affected by external factors. To analyze the influence of different stain carriers on the detectability of mRNA markers from bloodstains, this study examined 10 carriers, including a knife, cotton swab, paper, plastic, leather, cement, chopsticks, clothes, ceramic block, and wall. After detecting five specific mRNA markers (HBA, HBB, ALAS2, GYPA, and SPTB) and the housekeeping gene B2M in peripheral blood samples, no statistically significant differences in the effects of the carriers were found. The results suggest that when performing mRNA testing on bloodstains, the effect of the stain carrier has little influence.

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来源期刊

Journal of Forensic Science and Medicine Social Sciences-Law

CiteScore

0.40

自引率

0.00%

发文量

审稿时长

39 weeks