大戟根际链霉菌MB106高效抗菌活性物质的提取与鉴定

Dian Rachma Wijayanti, Almatin Puspa Dewi
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引用次数: 3

摘要

背景:放线菌是一类在药学和医学中发挥重要作用的细菌,它们能够以具有各种化学结构和生物活性的生物活性化合物的形式产生次级代谢产物。链霉菌属产生超过70%的放线菌天然产物。特别是,抗生素代表了最大的群体。通过突变,细菌可以在化疗中存活下来。因此,它的感染很难根除。因此,寻找新的强效生物活性化合物是重要的方法:分离制备、生产和提取生物活性化合物,粗提物抗菌试验,生物活性化合物的鉴定结果:每100µl粗提物具有抗菌活性。抑制区范围为11.5-13mm。正丁醇提取物中检测到多种生物活性化合物。链霉菌MB 106粗提物中检测到9种生物活性化合物。萘最高(43.89%)。最低的是3-亚氨基-3h,5h-[1,2,4]噻二唑[3,4-1]异吲哚(1.70%)。据报道,6种化合物具有抗菌活性丁烷、1,1-二丁氧基、萘、壬烷、二十二烷、二十烷和二十烷结论:粗提取物对大肠杆菌ATCC 8739的抑菌带平均为12mm。链霉菌MB 106中含有9种生物活性化合物。其中六种是有前景的候选药物。对这些化合物的进一步研究对未来的候选药物至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Extraction and Identification Potent Antibacterial Bioactive Compound of Streptomyces sp. MB 106 from Euphorbia sp. Rhizosphere
Background: Actinomycetes are groups of bacteria that play an important role in pharmacy and medicine in their ability to produce secondary metabolites in the form of bioactive compounds with various chemical structures and biological activities. The genus Streptomyces produces more than 70% of all described actinomycetes natural products. In particular, antibiotics represent the largest group. Through mutations, bacteria may survive chemotherapy. Thus its infection is hard to eradicate. Therefore the search for new potent bioactive compounds is important Methods: Isolate preparation, Production, and extraction bioactive compound, Crude extract antibacterial test, Identification of bioactive compound Results: There are antibacterial activities per 100 µl crude extract. Inhibition zone range from 11.5 to 13 mm. Various bioactive compounds detected in n-Butanol extract, There are nine bioactive compounds detected from Streptomyces sp. MB 106 crude extract. The highest was Naphthalene (43.89 %). The lowest was 3-Imino-3h,5h- [1,2,4] Thiadiazolo [3,4-1] Isoindole (1.70 %). Six compounds were reported to have antimicrobial activities butane, 1,1-dibutoxy, naphthalene, nonadecane, docosane, heneicosane, and eicosane Conclusions: Crude extract showed an average of 12 mm inhibition zone against Escherichia coli ATCC 8739. There are nine bioactive compounds from Streptomyces sp. MB 106. Six of them are promising drugs candidate. Further studies on these compounds are essential for future drugs candidate.
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