Effect of Cytokinins on the Vegetative Propagation of Olea europaea L. (cv. Koroneiki)

The study was carried out in soil born diseases laboratory, Iranian Research Institute of Plant Protection during Mar 2022 to Sep 2022. The Single node explants (prepared from young certificated scions from Fadak garden in Qom province) of Koroneiki olive scions cultured on DKW medium. Culture media were free from PGRs in establishment stage. After the mentioned stage, explant cultured on DKW media fortified with PGRs, in order to presentation a simple and repeatable method for shoot multiplication which is one of the important method for In Vitro olive propagation by examining three item included, the mean shoot length, the mean shoot number and the mean leaf number per explants. PGRs included 1 – 4 mg/l ZEA, KIN, 2ip, TDZ and BA. ZEA and KIN to be superior to other cytokinin in terms of mean shoot length and mean shoot numbers, respectively. All of investigated item especially the mean shoot number and the mean shoot length increased when the concentrations of PGRs increased. The influence of other cytokines (2ip, BA and TDZ) on three above investigated items was different. Supplemented DKW media with 4 mg/l 2ip resulted the maximum number leaf per explants. Callusing and low growth of micro shoots on explants were the dominant responses on DKW media plus 3 mg/l BA. In addition, few weak micro shoots regenerated on DKW media with 4 mg/l TDZ.