脯氨酸-丙氨酸-丝氨酸肽与聚乙二醇和聚甘油生物相容性的体外评价

IF 1.5 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Peptide Science Pub Date : 2023-08-11 DOI:10.1002/pep2.24330

Qianyu Zhang, Hongjing Chen, Huali Chen

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引用次数: 0

摘要

聚乙二醇化的实质是利用由脯氨酸、丙氨酸和丝氨酸组成的多肽对药物进行亲水性修饰。在这项工作中，我们重点研究了两种PAS肽(PAS)8和(PA3)7以及更常用的聚合物聚乙二醇(PEG)和聚甘油(PG)的生物相容性评价。本研究证实(PAS)8和(PA3)7对HUVEC和RAW 264.7细胞株均表现出低细胞毒性。红细胞溶血和凝集也可以忽略不计，这表明血液相容性良好。它们与牛血清白蛋白的潜在相互作用也被研究，结果表明聚合物与蛋白质之间几乎没有疏水相互作用。综上所述，(PAS)8和(PA3)7以及PEG和PG在这些研究中均表现出相当的相容性和安全性，提示(PAS)8和(PA3)7可作为未来研究中PEG替代的潜在候选物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

An in vitro evaluation of the biocompatibility of proline‐alanine‐serine peptides compared with polyethylene glycol and polyglycerol

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An in vitro evaluation of the biocompatibility of proline‐alanine‐serine peptides compared with polyethylene glycol and polyglycerol

PASylation has been recently reported as a feasible alternative to PEGylation, which in essence is using polypeptides constituted of a combination of proline, alanine and serine for the hydrophilic modification of pharmaceuticals. In this work, we focused on the biocompatibility evaluation of two PAS peptides, (PAS)8 and (PA3)7 as well as the more frequently used polymers polyethylene glycol (PEG) and polyglycerol (PG). It has been verified in this study that (PAS)8 and (PA3)7 both exhibited low cell toxicity against HUVEC and RAW 264.7 cell lines. They also showed negligible RBC hemolysis and agglutination, which demonstrated adequate hemocompatibility. Their potential interactions with bovine serum albumin have also been investigated, and the results indicated little hydrophobic interactions between the polymers and protein. In conclusion, (PAS)8 and (PA3)7 as well as PEG and PG all showed considerable compatibility and safety in these studies, suggesting that (PAS)8 and (PA3)7 could be considered as potential candidates for PEG replacement in future studies.

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来源期刊

Peptide Science Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

5.20

自引率

4.20%

发文量

期刊介绍： The aim of Peptide Science is to publish significant original research papers and up-to-date reviews covering the entire field of peptide research. Peptide Science provides a forum for papers exploring all aspects of peptide synthesis, materials, structure and bioactivity, including the use of peptides in exploring protein functions and protein-protein interactions. By incorporating both experimental and theoretical studies across the whole spectrum of peptide science, the journal serves the interdisciplinary biochemical, biomaterials, biophysical and biomedical research communities. Peptide Science is the official journal of the American Peptide Society.