OR2W3在胃腺癌中的表达及意义

中国综合临床 Pub Date : 2019-11-01 DOI:10.3760/CMA.J.ISSN.1008-6315.2019.06.006

Aidong Liu, Shifei Liu, Jinhui Pang, Feng Yan

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引用次数: 0

摘要

目的检测和分析嗅受体家族2亚家族W成员3（OR2W3）在胃腺癌中的表达及其意义，探讨其与细胞增殖和凋亡的关系。方法选择2013年1月至2014年6月在华南理工大学附属医院确诊并接受根治性手术的61例胃腺癌患者作为研究对象。选择肿瘤组织作为观察组，选择正常胃粘膜（61例）作为对照组。免疫组化检测两组or2v3和HER-2的表达。用Fish法检测部分HER-2基因表达不确定的患者的HER-2基因扩增情况。观察组用Western blot法检测PCNA和BAX的表达。结果观察组OR2W3阳性率（50.8%，31/61）高于对照组（8.2%（5/61）），两组差异有统计学意义（χ2=26.63，P<0.05），OR2W3在最大直径（≥6cm）为68.8%（22/32），<6cm为31.0%（9/29），少浆膜25.0%（5/20），血管和淋巴管受累（受累78.6%（11/14），无受累42.6%（20/47）），淋巴结转移（转移82.1%（23/28），无转移24.2%（3/33））和TNM分期（Ⅲ+Ⅳ78.1%（25/32），观察组Ⅰ+Ⅱ表达率为20.7%（6/29），差异有统计学意义（χ2分别为8.423、7.937、5.559、20.318、20.080，P均<0.05），OR2W3表达与生存时间呈正相关（X2=5.31，P均＜0.05），与PCNA表达呈正相关（r=0.54，P＜0.05）HER-2阳性者例，HER-2阴性者53例。结论胃癌组织中OR2W3表达显著增加，可促进癌症细胞增殖，抑制细胞凋亡。OR2W3与HER-2的表达有关。OR2W3表达的检测可能对判断预后有一定价值。关键词：胃腺癌；或2w3；扩散；细胞凋亡；预后

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Expression and significance of OR2W3 in gastric adenocarcinoma

Objective To detect and analyze the expression and significance of olfactory receptor family 2 subfamily W member 3 (OR2W3) in gastric adenocarcinoma, and to explore its correlation with cell proliferation and apoptosis. Methods From January 2013 to June 2014, 61 patients with gastric adenocarcinoma diagnosed in the Affiliated Hospital of North China University of Technology and undergoing radical operation were selected as the study subjects.Neoplasm tissue were selected as the observation group, normal gastric mucosa(61 cases) were selected as the control group.Immunohistochemistry was used to detect the expression of or2w3 and HER-2 in the two groups.Fish was used to detect the amplification of HER-2 gene in some of the patients with uncertain expression of HER-2.Western blot was used to detect the expression of PCNA and BAX in the observation group. Results Positive rate of OR2W3 was higher in the observation group(50.8%, 31/61) than that in the control group(8.2%(5/61)), and the difference between the two groups was statistically significant(χ2=26.63, P<0.05). The difference of the positive rate of OR2W3 in the maximum diameter(≥6 cm was 68.8%(22/32), <6 cm was 31.0%(9/29)), depth of invasion(seroas and beyond was 63.4%(26/41), less serosa was 25.0%(5/20)), vascular and lymphatic vessel involvement(involvement was 78.6%(11/14), no involvement was 42.6%(20/47)), lymph node metastasis(metastasis was 82.1%(23/28), no metastasis was 24.2%(3/33)) and TNM staging(Ⅲ+ Ⅳ was 78.1%(25/32), Ⅰ+ Ⅱ was 20.7%(6/29)) in observation group was statistically significant(χ2 was 8.423, 7.937, 5.559, 20.318, 20.080, respectively, all P<0.05). Expression of OR2W3 was correlated with survival time(X2=5.31, P<0.05). Positive correlation was found between OR2W3 and PCNA expression(r=0.54, P<0.05). Negative correlation were found between OR2W3 and BAX expression(r=-0.59, P<0.05). There were 8 cases of HER-2 positive and 53 cases of HER-2 negative.The difference of OR2W3 expression in tumors with different HER-2 expression was statistically significant (χ2=4.957, P<0.05). Conclusion The expression of OR2W3 in gastric adenocarcinoma tissue is significantly increased, which can promote the proliferation of cancer cells and inhibit apoptosis.OR2W3 is related to HER-2 expression.Detection of OR2W3 expression may be of some value in judging prognosis. Key words: Gastric adenocarcinoma; OR2W3; Proliferation; Apoptosis; Prognosis

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来源期刊

中国综合临床

CiteScore

0.10

自引率

0.00%

发文量

16855

期刊介绍： Clinical Medicine of China is an academic journal organized by the Chinese Medical Association (CMA), which mainly publishes original research papers, reviews and commentaries in the field. Clinical Medicine of China is a source journal of Peking University (2000 and 2004 editions), a core journal of Chinese science and technology, an academic journal of RCCSE China Core (Extended Edition), and has been published in Chemical Abstracts of the United States (CA), Abstracts Journal of Russia (AJ), Chinese Core Journals (Selection) Database, Chinese Science and Technology Materials Directory, Wanfang Database, China Academic Journal Database, JST Japan Science and Technology Agency Database (Japanese) (2018) and other databases.