C. Ut, C. Berbegal, V. Lizama, L. Polo, M.J. García, L. Andrés, I. Pardo, I. Álvarez
{"title":"产自西班牙乌蒂尔- requena的“帕戈”梅洛葡萄酒中酿酒酵母菌的分离与鉴定","authors":"C. Ut, C. Berbegal, V. Lizama, L. Polo, M.J. García, L. Andrés, I. Pardo, I. Álvarez","doi":"10.1111/ajgw.12536","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background and Aims</h3>\n \n <p>To achieve both the control of the undesirable yeasts and the improvement of the characteristics of Merlot ‘Pago’ wines, <i>Saccharomyces cerevisiae</i> yeast diversity was investigated in a spontaneous ‘Pago’ Merlot fermentation from the Utiel-Requena region (Spain). Isolates of <i>S. cerevisiae</i> were characterised by a holistic procedure using the same Merlot grape must from which they were isolated.</p>\n </section>\n \n <section>\n \n <h3> Methods and Results</h3>\n \n <p>Yeasts were identified by internal transcribed spacer analysis and typed by <i>Hinf</i>I mDNA restriction analysis. Growth and metabolic characteristics of the isolates were determined by laboratory-scale (50 L) fermentations of Merlot must and the composition of the polyphenolic and the volatile compounds and the sensory attributes of the wines were determined. Twelve <i>S. cerevisiae</i> strains were isolated and characterised. Strains 2E, 4A, 7A and 7F had better growth characteristics. Strains 9C and 7F produced wines of good intensity and colour quality, marked intensity and aroma quality, fruity character and better overall quality. Strain 9C displayed poor growth.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Strain 7F combined good growth characteristics and produced Merlot wines with the best colour, aroma and flavour characteristics during microvinifications.</p>\n </section>\n \n <section>\n \n <h3> Significance of the Study</h3>\n \n <p>Characterisation of <i>S. cerevisiae</i> made entirely in Merlot grape must allowed the influence of yeast strains on the final characteristics of commercial-scale Merlot ‘Pago’ wines to be more accurately deduced.</p>\n </section>\n </div>","PeriodicalId":8582,"journal":{"name":"Australian Journal of Grape and Wine Research","volume":"28 3","pages":"330-346"},"PeriodicalIF":2.5000,"publicationDate":"2021-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ajgw.12536","citationCount":"2","resultStr":"{\"title\":\"Isolation and characterisation of autochthonous Saccharomyces cerevisiae from ‘Pago’ Merlot wines of Utiel-Requena (Spain) origin\",\"authors\":\"C. Ut, C. Berbegal, V. Lizama, L. Polo, M.J. García, L. Andrés, I. Pardo, I. Álvarez\",\"doi\":\"10.1111/ajgw.12536\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background and Aims</h3>\\n \\n <p>To achieve both the control of the undesirable yeasts and the improvement of the characteristics of Merlot ‘Pago’ wines, <i>Saccharomyces cerevisiae</i> yeast diversity was investigated in a spontaneous ‘Pago’ Merlot fermentation from the Utiel-Requena region (Spain). Isolates of <i>S. cerevisiae</i> were characterised by a holistic procedure using the same Merlot grape must from which they were isolated.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods and Results</h3>\\n \\n <p>Yeasts were identified by internal transcribed spacer analysis and typed by <i>Hinf</i>I mDNA restriction analysis. Growth and metabolic characteristics of the isolates were determined by laboratory-scale (50 L) fermentations of Merlot must and the composition of the polyphenolic and the volatile compounds and the sensory attributes of the wines were determined. Twelve <i>S. cerevisiae</i> strains were isolated and characterised. Strains 2E, 4A, 7A and 7F had better growth characteristics. Strains 9C and 7F produced wines of good intensity and colour quality, marked intensity and aroma quality, fruity character and better overall quality. Strain 9C displayed poor growth.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>Strain 7F combined good growth characteristics and produced Merlot wines with the best colour, aroma and flavour characteristics during microvinifications.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Significance of the Study</h3>\\n \\n <p>Characterisation of <i>S. cerevisiae</i> made entirely in Merlot grape must allowed the influence of yeast strains on the final characteristics of commercial-scale Merlot ‘Pago’ wines to be more accurately deduced.</p>\\n </section>\\n </div>\",\"PeriodicalId\":8582,\"journal\":{\"name\":\"Australian Journal of Grape and Wine Research\",\"volume\":\"28 3\",\"pages\":\"330-346\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2021-11-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ajgw.12536\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Australian Journal of Grape and Wine Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/ajgw.12536\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Australian Journal of Grape and Wine Research","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/ajgw.12536","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Isolation and characterisation of autochthonous Saccharomyces cerevisiae from ‘Pago’ Merlot wines of Utiel-Requena (Spain) origin
Background and Aims
To achieve both the control of the undesirable yeasts and the improvement of the characteristics of Merlot ‘Pago’ wines, Saccharomyces cerevisiae yeast diversity was investigated in a spontaneous ‘Pago’ Merlot fermentation from the Utiel-Requena region (Spain). Isolates of S. cerevisiae were characterised by a holistic procedure using the same Merlot grape must from which they were isolated.
Methods and Results
Yeasts were identified by internal transcribed spacer analysis and typed by HinfI mDNA restriction analysis. Growth and metabolic characteristics of the isolates were determined by laboratory-scale (50 L) fermentations of Merlot must and the composition of the polyphenolic and the volatile compounds and the sensory attributes of the wines were determined. Twelve S. cerevisiae strains were isolated and characterised. Strains 2E, 4A, 7A and 7F had better growth characteristics. Strains 9C and 7F produced wines of good intensity and colour quality, marked intensity and aroma quality, fruity character and better overall quality. Strain 9C displayed poor growth.
Conclusions
Strain 7F combined good growth characteristics and produced Merlot wines with the best colour, aroma and flavour characteristics during microvinifications.
Significance of the Study
Characterisation of S. cerevisiae made entirely in Merlot grape must allowed the influence of yeast strains on the final characteristics of commercial-scale Merlot ‘Pago’ wines to be more accurately deduced.
期刊介绍：
The Australian Journal of Grape and Wine Research provides a forum for the exchange of information about new and significant research in viticulture, oenology and related fields, and aims to promote these disciplines throughout the world. The Journal publishes results from original research in all areas of viticulture and oenology. This includes issues relating to wine, table and drying grape production; grapevine and rootstock biology, genetics, diseases and improvement; viticultural practices; juice and wine production technologies; vine and wine microbiology; quality effects of processing, packaging and inputs; wine chemistry; sensory science and consumer preferences; and environmental impacts of grape and wine production. Research related to other fermented or distilled beverages may also be considered. In addition to full-length research papers and review articles, short research or technical papers presenting new and highly topical information derived from a complete study (i.e. not preliminary data) may also be published. Special features and supplementary issues comprising the proceedings of workshops and conferences will appear periodically.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
