葡萄叶卷相关病毒3在红白葡萄中的体外生物学指标研究

IF 2.5 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
X.-Y. Hao, B.-L. Jiao, M.-R. Wang, Y.-L. Wang, B.-X. Shang, J.-Y. Wang, Q.-C. Wang, Y. Xu
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引用次数: 1

摘要

背景和目的病毒检测在需要病毒检测植物的项目中是必不可少的。本研究的目的是建立葡萄叶相关病毒-3 (glrav3)在红白葡萄中的体外生物学方法。方法与结果以健康的红莓葡萄赤霞珠[CaSa (H)]为砧木,对感染病毒的赤霞珠[CaSa (VI)]和霞多丽[Ch (VI)]接穗进行了微嫁接试验。连接砧木和接穗的维管束发育,移植后3周和4周成活率分别为100%。在CaSa (VI)和Ch (VI)接穗微嫁接后12周,80%和20%的CaSa (H)砧木分别表达了glrav3的症状。将CaSa (VI)和Ch (VI)嫁接5周后的砧木CaSa (H)在添加75 mmol/L NaCl的半强度Murashige和Skoog培养基上培养,诱导盐胁迫。盐胁迫5周后,88%的CaSa (VI)/CaSa (H)微移植物和85%的Ch (VI)/CaSa (H)微移植物表达glrav3症状。用CaSa (VI)接穗嫁接的CaSa (H)砧木叶片呈紫红色,叶片向下滚动;用Ch (VI)接穗嫁接的CaSa (H)砧木叶片向下滚动,叶片变黄。结论盐胁迫提高了葡萄glrav3的体外生物学指标。研究的意义体外微嫁接和盐胁迫的建立为红白葡萄glrav3的标引提供了替代方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

In vitro biological indexing of grapevine leafroll-associated virus 3 in red- and white-berried grapevines (Vitis vinifera)

In vitro biological indexing of grapevine leafroll-associated virus 3 in red- and white-berried grapevines (Vitis vinifera)

Background and Aims

Virus detection is essential in programs in which virus-tested plants are required. The aim of this study was to establish in vitro biological methods for indexing grapevine leafroll-associated virus-3 (GLRaV-3) in red- and white-berried grapevines.

Methods and Results

Healthy, red-berried grapevine Cabernet Sauvignon [CaSa (H)] was used as a rootstock in micrografting experiments with virus-infected Cabernet Sauvignon [CaSa (VI)] and Chardonnay [Ch (VI)] scions. Vascular bundles connecting the rootstock and scions developed, and 100% of the micrografts survived 3 and 4 weeks after micrografting, respectively. Symptoms of GLRaV-3 were expressed in 80 and 20% of CaSa (H) rootstocks micrografted with CaSa (VI) and with Ch (VI) scions, respectively, 12 weeks after micrografting. The rootstock CaSa (H) that had been micrografted with CaSa (VI) and Ch (VI) scions for 5 weeks was subsequently cultured on half-strength Murashige and Skoog medium supplemented with 75 mmol/L NaCl to induce salt stress. The GLRaV-3 symptoms were expressed in 88% of micrografts CaSa (VI)/CaSa (H) and 85% of micrografts Ch (VI)/CaSa (H) after 5 weeks of salt stress. Reddish-purple leaf coloration and downward rolling were observed in CaSa (H) rootstocks that were micrografted with CaSa (VI) scions, while leaf downward rolling and yellowing were seen in CaSa (H) rootstocks that were micrografted with Ch (VI) scions.

Conclusions

Salt stress improved in vitro biological indexing of GLRaV-3 in grapevines.

Significance of the Study

Establishment of in vitro micrografting and salt stress provided alternative methods for indexing of GLRaV-3 in red- and white-berried grapevines.

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来源期刊
CiteScore
5.30
自引率
7.10%
发文量
35
审稿时长
3 months
期刊介绍: The Australian Journal of Grape and Wine Research provides a forum for the exchange of information about new and significant research in viticulture, oenology and related fields, and aims to promote these disciplines throughout the world. The Journal publishes results from original research in all areas of viticulture and oenology. This includes issues relating to wine, table and drying grape production; grapevine and rootstock biology, genetics, diseases and improvement; viticultural practices; juice and wine production technologies; vine and wine microbiology; quality effects of processing, packaging and inputs; wine chemistry; sensory science and consumer preferences; and environmental impacts of grape and wine production. Research related to other fermented or distilled beverages may also be considered. In addition to full-length research papers and review articles, short research or technical papers presenting new and highly topical information derived from a complete study (i.e. not preliminary data) may also be published. Special features and supplementary issues comprising the proceedings of workshops and conferences will appear periodically.
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