Thatiana V. Soria, Thomaz M. C. Fabrin, Nathália A. Diamante, Thaís F. M. Mota, Isadora J. de Oliveira, Alessandra V. de Oliveira, Weferson J. da Graça, Sônia M. A. P. Prioli, Alberto J. Prioli
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In this study, a fragment of the mitochondrial Cytochrome c Oxidase subunit I (<i>COI</i>) gene was used to analyze the diversity of 10 morphospecies of <i>Gymnotus</i> (<i>G. carapo</i>, <i>G. pantanal</i>, <i>G. sylvius</i>, <i>G. ineaquilabiatus</i>, <i>G. pantherinus</i>, <i>G. coropinae</i>, <i>G. jonasi</i>, <i>G. cylindricus</i>, <i>G. chaviro</i>, <i>G. tigre</i>) in two Neotropical river basins (Paraná River and Doce River), in addition to specimens identified as <i>Gymnotus</i> sp. At least 15 OTUs were determined, eight of which belonged to the <i>carapo</i> complex. Our results indicate the existence of at least four <i>Gymnotus</i> species belonging to the <i>carapo</i> clade in the upper Paraná River basin and possibly erroneous previous identifications. Besides that, we identified a possible new species of <i>Gymnotus</i> in the Rio Doce basin. The Generalized Mixed Yule Coalescent (GMYC) and Poisson Tree Processes (PTP) molecular delimitation approaches place other specimens found in the upper Paraná River and Guaíba River (<i>Gymnotus</i> sp.) in well-established groups that should be considered in studies involving the <i>carapo</i> complex.</p>\n </section>\n </div>","PeriodicalId":54928,"journal":{"name":"International Review of Hydrobiology","volume":"103 5-6","pages":"120-126"},"PeriodicalIF":0.9000,"publicationDate":"2018-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/iroh.201801954","citationCount":"4","resultStr":"{\"title\":\"DNA barcoding analysis of Gymnotus species in two Neotropical river basins\",\"authors\":\"Thatiana V. Soria, Thomaz M. C. Fabrin, Nathália A. Diamante, Thaís F. M. Mota, Isadora J. de Oliveira, Alessandra V. de Oliveira, Weferson J. da Graça, Sônia M. A. P. Prioli, Alberto J. 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引用次数: 4
摘要
裸子鱼是新热带地区种类最丰富的电鱼属。最近,在这个属中发现了一种以前未知的多样性,激发了对新物种的追求。本研究利用线粒体细胞色素c氧化酶亚基I (COI)基因片段,分析了两个新热带河流流域(paran河和Doce河)的10个Gymnotus形态种(G. carapo, G. pantanal, G. sylvius, G. ineaquilabiatus, G. pantherinus, G. coropinae, G. jonasi, G. icus, G. chaviro, G. tigre)的多样性,并确定了至少15个OTUs,其中8个属于carapo复合物。我们的研究结果表明,在paran河上游流域存在至少4种属于甲壳目的裸子目,并且可能存在先前错误的鉴定。此外,我们还在Rio Doce盆地发现了一种可能的Gymnotus新种。广义混合Yule聚结(GMYC)和泊松树过程(PTP)分子划分方法将在paran河上游和Guaíba河(Gymnotus sp.)中发现的其他标本置于已建立的类群中,这些类群应在涉及carapo复合体的研究中加以考虑。
DNA barcoding analysis of Gymnotus species in two Neotropical river basins
Gymnotus is the most species rich genus of electric fish in the Neotropical region. Recently, a previously unknown diversity has been found in this genus, motivating a pursuit for new species. In this study, a fragment of the mitochondrial Cytochrome c Oxidase subunit I (COI) gene was used to analyze the diversity of 10 morphospecies of Gymnotus (G. carapo, G. pantanal, G. sylvius, G. ineaquilabiatus, G. pantherinus, G. coropinae, G. jonasi, G. cylindricus, G. chaviro, G. tigre) in two Neotropical river basins (Paraná River and Doce River), in addition to specimens identified as Gymnotus sp. At least 15 OTUs were determined, eight of which belonged to the carapo complex. Our results indicate the existence of at least four Gymnotus species belonging to the carapo clade in the upper Paraná River basin and possibly erroneous previous identifications. Besides that, we identified a possible new species of Gymnotus in the Rio Doce basin. The Generalized Mixed Yule Coalescent (GMYC) and Poisson Tree Processes (PTP) molecular delimitation approaches place other specimens found in the upper Paraná River and Guaíba River (Gymnotus sp.) in well-established groups that should be considered in studies involving the carapo complex.
期刊介绍:
As human populations grow across the planet, water security, biodiversity loss and the loss of aquatic ecosystem services take on ever increasing priority for policy makers. International Review of Hydrobiology brings together in one forum fundamental and problem-oriented research on the challenges facing marine and freshwater biology in an economically changing world. Interdisciplinary in nature, articles cover all aspects of aquatic ecosystems, ranging from headwater streams to the ocean and biodiversity studies to ecosystem functioning, modeling approaches including GIS and resource management, with special emphasis on the link between marine and freshwater environments. The editors expressly welcome research on baseline data. The knowledge-driven papers will interest researchers, while the problem-driven articles will be of particular interest to policy makers. The overarching aim of the journal is to translate science into policy, allowing us to understand global systems yet act on a regional scale.
International Review of Hydrobiology publishes original articles, reviews, short communications, and methods papers.