Antioxidant Study of Coffea Cruda

Background: Coffea cruda (family Rubiaceae) has great therapeutic significance in Indian system of medicine due to its rich antioxidant activity. Coffea cruda is the major source of antioxidant compounds as it has strong ability to inhibit the process of oxidation in the body which attributes to its high antioxidant activity. The main objective of the present work was to evaluate antioxidant study of Coffea cruda alcoholic extract. Objectives:The present study was designed to investigate the HPTLC study and anti-oxidant activity of of Coffea cruda alcoholic extract. Materials and methods: Anti-oxidant activity of alcoholic extract of Coffea cruda was determined by DPPH free radical scavenging activity, Total phenol, Total flavonoid content, ABTS and FRAP assay methods. Whereas, HPTLC study performed on precoated silica gel 60 F254 TLC plate where, mobile phase used was toluene: acetone (7:3, v/v). HPTLC UV detection was performed at 254 nm. Results:The result revealed that different antioxidant assays of alcoholic extract of Coffea cruda seed shows prominent antioxidant activity and High performance thin layer chromatography study indicates the presence of alkaloid compound caffeine in chloroform extract of Coffea cruda. Conclusion:The present study justifies Coffea cruda medicinal usage in traditional medicines. High antioxidant potentials are the reason for its cure and healing properties.