冲绳斑蝰（Ovophis okinavensis）多态性微卫星标记的建立

IF 0.6 4区生物学 Q4 ZOOLOGY

Current Herpetology Pub Date : 2019-08-31 DOI:10.5358/HSJ.38.169

Yohei Kadota, Kazuki Kurita, E. Inoue

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引用次数: 0

摘要

我们分离并鉴定了琉球列岛特有的蝰蛇Ovophis okinavensis的11个微卫星标记。这些标记在冲绳岛的25个个体中显示出多态性。每个基因座的等位基因数为3-11，观察到的和预期的杂合度分别为0.240-0.960和0.218-0.853。交叉扩增证实，11个微卫星标记中至少有7个适用于一个推定的亲缘关系，即纤细Trimereurus gracilis。这里提供的标记预计不仅对O.okinavensis及其亲属的群体和/或个体水平的遗传研究有价值。

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Development of Polymorphic Microsatellite Markers for the Okinawa Pit Viper, Ovophis okinavensis

We isolated and characterized 11 microsatellite markers for Ovophis okinavensis, a viperid species endemic to the Ryukyu Archipelago. These markers showed polymorphism among 25 individuals from Okinawajima Island. The number of alleles per locus was 3–11, and observed and expected heterozygosity were 0.240–0.960 and 0.218–0.853, respectively. Cross-amplification confirmed that at least seven out of the 11 microsatellite markers were applicable to a putative relative, Trimeresurus gracilis. The markers provided here are expected to be valuable for population- and/or individual-level genetic studies of not only O. okinavenesis, but also its relatives.

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来源期刊

Current Herpetology Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

1.20

自引率

14.30%

发文量

期刊介绍： Current Herpetology publishes original research articles on amphibians and reptiles. It is the official journal of the Herpetological Society of Japan and is a continuation of Acta Herpetologica Japonica (1964–1971) and Japanese Journal of Herpetology (1972-1999).