解淀粉芽孢杆菌和暹罗芽孢杆菌的抗菌化合物:筛选、表征和评价

R. Vasait, S. Bhamare, Sayali Jamdhade, Yogita Savkar
{"title":"解淀粉芽孢杆菌和暹罗芽孢杆菌的抗菌化合物:筛选、表征和评价","authors":"R. Vasait, S. Bhamare, Sayali Jamdhade, Yogita Savkar","doi":"10.21448/ijsm.1258717","DOIUrl":null,"url":null,"abstract":"This study was aimed at isolating potential antimicrobial compound (AMC) producing bacteria. AMC produced by a Bacillus species was evaluated further for its antimicrobial potential. Antimicrobial compound-producing bacteria were isolated from the soil of crop fields from the local region of Satana, Nashik (India), and tested against clinical isolates. Both isolates exhibited remarkable antibacterial potential against Gram-positive and Gram-negative clinical isolates. The AMCs of both SYS 1 and SYS 2 exhibited excellent antibacterial activity against Salmonella paratyphi B and Staphylococcus aureus. Both AMC-producing isolates were characterized and identified. Bacillus species SYS 1 and SYS 2 were identified as Bacillus amyloliquefaciens SYS 1 and Bacillus siamensis SYS 2, respectively. The highest antimicrobial activity of AMC produced by Bacillus amyloliquefaciens SYS 1 was exhibited against Salmonella paratyphi B (28 mm), followed by Staphylococcus aureus (26 mm). Bacillus siamensis SYS 2 AMC extracted by the solvent ethyl acetate exerted the highest antimicrobial activity against Salmonella paratyphi B (18 mm), followed by Staphylococcus aureus (16 mm). A partial characterization of the AMC was conducted and evaluated to contain amino acids and proteins. A higher total protein content of 17.9 μg/mL was estimated in the partially purified AMC of Bacillus amyloliquefaciens SYS 1. A detailed evaluation of the structural characteristics of AMC could prove its importance in commercial applications.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antibacterial compound of Bacillus Amyloliquefaciens and Bacillus Siamensis: screening, characterization, and evaluation\",\"authors\":\"R. Vasait, S. Bhamare, Sayali Jamdhade, Yogita Savkar\",\"doi\":\"10.21448/ijsm.1258717\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study was aimed at isolating potential antimicrobial compound (AMC) producing bacteria. AMC produced by a Bacillus species was evaluated further for its antimicrobial potential. Antimicrobial compound-producing bacteria were isolated from the soil of crop fields from the local region of Satana, Nashik (India), and tested against clinical isolates. Both isolates exhibited remarkable antibacterial potential against Gram-positive and Gram-negative clinical isolates. The AMCs of both SYS 1 and SYS 2 exhibited excellent antibacterial activity against Salmonella paratyphi B and Staphylococcus aureus. Both AMC-producing isolates were characterized and identified. Bacillus species SYS 1 and SYS 2 were identified as Bacillus amyloliquefaciens SYS 1 and Bacillus siamensis SYS 2, respectively. The highest antimicrobial activity of AMC produced by Bacillus amyloliquefaciens SYS 1 was exhibited against Salmonella paratyphi B (28 mm), followed by Staphylococcus aureus (26 mm). Bacillus siamensis SYS 2 AMC extracted by the solvent ethyl acetate exerted the highest antimicrobial activity against Salmonella paratyphi B (18 mm), followed by Staphylococcus aureus (16 mm). A partial characterization of the AMC was conducted and evaluated to contain amino acids and proteins. A higher total protein content of 17.9 μg/mL was estimated in the partially purified AMC of Bacillus amyloliquefaciens SYS 1. A detailed evaluation of the structural characteristics of AMC could prove its importance in commercial applications.\",\"PeriodicalId\":14437,\"journal\":{\"name\":\"International Journal of Secondary Metabolite\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-06-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Secondary Metabolite\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21448/ijsm.1258717\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Secondary Metabolite","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21448/ijsm.1258717","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

本研究旨在分离潜在的抗微生物化合物(AMC)产生菌。进一步评估了由芽孢杆菌产生的AMC的抗菌潜力。从印度纳希克萨塔纳当地的农田土壤中分离出产生抗菌化合物的细菌,并对临床分离株进行了测试。两个分离株对革兰氏阳性和革兰氏阴性临床分离株均表现出显著的抗菌潜力。SYS1和SYS2的AMC对副伤寒沙门氏菌B和金黄色葡萄球菌都表现出优异的抗菌活性。对两个产生AMC的分离株进行了鉴定。芽孢杆菌属SYS1和SYS2分别鉴定为解淀粉芽孢杆菌SYS1和siamensis芽孢杆菌SYS2。解淀粉芽孢杆菌SYS1产生的AMC对副伤寒沙门氏菌B(28毫米)的抗菌活性最高,其次是金黄色葡萄球菌(26毫米)。用乙酸乙酯提取的siamensis芽孢杆菌SYS2 AMC对副伤寒沙门氏菌B(18mm)的抗菌活性最高,其次是金黄色葡萄球菌(16mm)。对AMC进行部分表征,并评估其含有氨基酸和蛋白质。在解淀粉芽孢杆菌SYS1的部分纯化的AMC中估计总蛋白含量较高,为17.9μg/mL。对AMC结构特征的详细评估可以证明其在商业应用中的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antibacterial compound of Bacillus Amyloliquefaciens and Bacillus Siamensis: screening, characterization, and evaluation
This study was aimed at isolating potential antimicrobial compound (AMC) producing bacteria. AMC produced by a Bacillus species was evaluated further for its antimicrobial potential. Antimicrobial compound-producing bacteria were isolated from the soil of crop fields from the local region of Satana, Nashik (India), and tested against clinical isolates. Both isolates exhibited remarkable antibacterial potential against Gram-positive and Gram-negative clinical isolates. The AMCs of both SYS 1 and SYS 2 exhibited excellent antibacterial activity against Salmonella paratyphi B and Staphylococcus aureus. Both AMC-producing isolates were characterized and identified. Bacillus species SYS 1 and SYS 2 were identified as Bacillus amyloliquefaciens SYS 1 and Bacillus siamensis SYS 2, respectively. The highest antimicrobial activity of AMC produced by Bacillus amyloliquefaciens SYS 1 was exhibited against Salmonella paratyphi B (28 mm), followed by Staphylococcus aureus (26 mm). Bacillus siamensis SYS 2 AMC extracted by the solvent ethyl acetate exerted the highest antimicrobial activity against Salmonella paratyphi B (18 mm), followed by Staphylococcus aureus (16 mm). A partial characterization of the AMC was conducted and evaluated to contain amino acids and proteins. A higher total protein content of 17.9 μg/mL was estimated in the partially purified AMC of Bacillus amyloliquefaciens SYS 1. A detailed evaluation of the structural characteristics of AMC could prove its importance in commercial applications.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
International Journal of Secondary Metabolite
International Journal of Secondary Metabolite Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
1.40
自引率
0.00%
发文量
28
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信