微环境对肺癌上皮-间质转化发展的影响

R. B. Kondratyuk, I. S. Grekov, E. A. Seleznev
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引用次数: 0

摘要

的相关性。上皮间充质转化(Epithelial-mesenchymal transformation, EMT)在很大程度上决定了不同部位癌症的生物学行为和预后。众所周知,在上皮表型向间质表型转变的控制和实现中起决定性作用的是微环境。同时,基质元素的组织化学和显微特征尚不清楚;因此,我们的研究目的是建立影响肺癌EMT发展的基质形态学特征。材料与方法。我们研究了32例肺癌,用苏木精和伊红染色切片,pH 1.0和2.5下的阿利新蓝,PAS反应,以及HMW, AE 1 / AE 3,细胞角蛋白18,c- erbb b2, vimentin E-cadherin, α -平滑肌肌动蛋白,GFAP和嗜铬粒蛋白A, Ki-67的单克隆抗体进行免疫组织化学研究。16例可见广泛的上皮-间质转化(EMT), 14例为局灶性转化,2例未见上皮-间质转化。在一半的广泛的紧急医疗救护案例中,它是完整的;在局灶性EMT病例中,不完全。基质的激活促进了从上皮到间充质表型的转变,即肌成纤维细胞的存在和细胞外基质的嗜酸性,炎症细胞的浸润,基质细胞中表皮生长因子受体(c- erbb b2)的表达,基质细胞的基质细胞的增殖和神经源性分化。结果和讨论。激活的基质与EMT的频率相关。因此,大面积活化基质与肌成纤维细胞和细胞外基质嗜酸症的存在在广泛性EMT(16- 87.5%中的14例)中比在局灶性EMT(14 - 28.6%中的4例)中更常见。差异有统计学意义,p < 0.01。炎症细胞浸润是转化信号的来源,基质细胞中表皮生长因子受体(c- erbb b2)的表达、基质细胞的增殖和神经源性分化也与EMT的发生频率相关。在所有病例中,差异均有统计学意义,p0.01。结论。所获得的数据表明,来自活化基质的信号对肿瘤细胞上皮-间质转化的发展有不容置疑的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microenvironment influence on the development of epithelial-mesenchymal transformation in lung cancer
Relevance. Epithelial-mesenchymal transformation (EMT) largely determines the biological behavior and prognosis of cancers of various localizations. It is known that the determining role in the control and implementation of the transition of the epithelial phenotype to the mesenchymal belongs to the microenvironment. At the same time, the histochemical and microscopic characteristics of stromal elements remain unclear; therefore, the aim of our study was to establish the morphological features of the stroma that affect the development of EMT in lung cancer. Materials and Methods. We studied 32 cases of lung cancer with hematoxylin and eosin staining of sections, Alcian blue at pH 1.0 and 2.5, PAS reaction, as well as immunohistochemical studies with monoclonal antibodies to HMW, AE 1 / AE 3, cytokeratin 18, c-erb B 2, vimentin E-cadherin, alpha-smooth muscle actin, GFAP and chromogranin A, Ki-67. In 16 cases, there was widespread epithelial-mesenchymal transformation (EMT), in 14 cases - focal and in 2 cases EMT was absent. In half of the cases of widespread EMT, it was complete; among the cases of focal EMT, it was not complete. The transition from the epithelial to the mesenchymal phenotype was facilitated by the activation of the stroma, namely, the presence of myofibroblasts and alcianophilia of the extracellular matrix, inflammatory cell infiltration, expression of the epidermal growth factor receptor (c-erb B 2) in stromal cells, proliferation and neurogenic differentiation of stromal cells of stromal cells. Results and Discussion. The activated stroma correlates with the frequency of EMT. Thus, large areas of activated stroma with the presence of myofibroblasts and alcianophilia of the extracellular matrix are more common in cases of widespread EMT (14 out of 16-87.5 %) than in cases of focal EMT (4 out of 14-28.6 %). The differences are statistically significant, p 0.01. Inflammatory cell infiltration, which is the source of a signal for transformation, expression of the epidermal growth factor receptor (c-erb B 2) in stromal cells, proliferation, and neurogenic differentiation of stromal cells also correlated with the frequency of EMT. In all cases, the differences are statistically significant, p0.01. Conclusions. The data obtained indicate the undoubted influence of signals from the activated stroma on the development of epithelial-mesenchymal transformation of tumor cells.
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