Effects of Plant Growth Regulators on Shoot Multiplication of Crepis bithynica (Asteraceae): An Endangered Rare Species in Türkiye

Aim of study: In this study, the effects of plant growth regulators (PGRs) on shoot multiplication of Crepis bithynica Boiss. (Asteraceae), an endangered rare species in Türkiye were investigated. Area of study: The plant samples were collected from Kastamonu Ilgaz Mountain National Park. Material and method: The nodal segments were used as explant and they were cultured on Murashige and Skoog (MS) basal media supplemented with different plant growth regulators. Morphological changes of propagated plants were monitored during 21 days in vitro cultures. Main results: All plant growth regulators induced shoot growth and development without callus formation. Additionally, the highest shoot number and length of shoot per explant were achieved in MS media supplemented with 4.4 µM 6-BA plus 0.5 µM NAA, and 4.9 µM 2iP plus 0.5 µM IBA, respectively. Highlights: This study is the first study on the effects of PGRs in shoot multiplication of C. bithynica, and the results obtained can be used micropropagation for the species.