墨西哥南下加利福尼亚leon Nodipecten subnodosus手蛤1型疱疹病毒

IF 0.5 Q4 BIOLOGY
C. Escobedo-Fregoso, A.Q. Alvarado-Arellano, J. A. Hernández-González, G. Mendoza Carrión, R. Vázquez-Juárez
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引用次数: 0

摘要

本研究首次报道了OsHV-1在墨西哥南下加利福尼亚州的扇贝中的存在;该回顾性研究旨在评估OsHV-1是否与2010年的人口下降有关。通过PCR检测病毒,并通过qPCR定量病毒颗粒。通过对用于鉴定OsHV-1变体的基因组的三个不同区域进行PCR扩增来进行OsHV-1检测。系统发育分析表明,在不同国家和双壳类先前采集的OsHV-1样本中,N.subnodusus OsHV-1的系统发育位置。系统发育分析表明,亚齿猪笼草的OsHV-1与OsHV-1μVar之间存在差异。相似性分析表明,Crassostrea gigas的OsHV-1与墨西哥的N.subodosus的OsHV1存在差异,但序列与1993年法国的OsHV-2相同。这项研究有助于更好地了解OsHV-1的遗传多样性,并确定病毒的潜在宿主或参与病毒周期的物种。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Herpesvirus de los ostreidos tipo 1 en la almeja mano de león Nodipecten subnodosus de Baja California Sur, México
This study is the first report of the presence of Ostreid herpes virus 1 (OsHV-1) in the scallop Nodipecten subnodosus from Baja California Sur, Mexico; collected from 2010 to 2012.The retrospective study was conducted to evaluate whether OsHV-1 was implicated in the population decline of 2010. The virus was detected by PCR, and viral particles were quantified by qPCR. The OsHV-1 detection was conducted by PCR amplification of three different regions of the genome used to identify OsHV-1 variants. A phylogenetic analysis indicated the phylogenetic position of N. subnodusus OsHV-1 among OsHV-1 samples previously collected in different countries and bivalves. Phylogeny analyses indicated differences between OsHV-1 of N. subnodosus and OsHV-1 μVar. Similarity analyses showed differences between the OsHV-1 of Crassostrea gigas and the OsHV-1 of N. subnodosus from Mexico, however the sequence was identical to that of OsHV-1 from France 1993. This study contributes to better understand the genetic diversity of OsHV-1 and to identify potential host of the virus or species involved in the viral cycle.
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