人参皂苷Rg3治疗急性放射性直肠炎大鼠模型的实验研究

Tingting Hu, Hongyi Cai
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引用次数: 1

摘要

目的评价人参皂苷Rg3(GRg3)治疗大鼠急性放射性直肠炎的疗效。方法在21.5Gy照射后第7天,将100只大鼠分为对照组(生理盐水,n=20)、GRg3组(灌胃GRg3 20mg/kg、40mg/kg和80mg/kg,每剂量n=20)和思密达混合灌肠组(n=20)。2周后,用水合氯醛(3ml/kg)麻醉所有大鼠,并在处死前从腹主动脉采集约5ml血样。取直肠组织进行Bax和NF-κB含量检测和HE染色,观察直肠组织的病理变化。离心后,收集上清液进行ELISA检测各组血清IL-2、IL-6、TNF-α和MPO水平。结果GRg3组大鼠血清炎性细胞因子、血清MPO浓度、肠组织Bax和NF-κB浓度均随GRg3剂量的增加而降低。大剂量GRg3组的这些参数明显低于生理盐水组(均P 0.05),表明GRg3对大鼠急性放射性直肠炎模型有良好的治疗作用。结论GRg3能显著降低急性放射性直肠炎模型大鼠肠组织中炎性细胞因子、Bax和NF-κB的浓度,其作用优于生理盐水。GRg3可用于治疗大鼠急性放射性直肠炎模型。关键词:人参皂苷Rg3;急性放射性直肠炎;大鼠
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Experimental study of ginsenoside Rg3 in the treatment of acute radiation proctitis in rat models
Objective To evaluate the efficacy of ginsenoside Rg3(GRg3) in the treatment of acute radiation proctitis in rat models. Methods On the 7th day after 21.5 Gy irradiation, 100 rats were divided into the control group (normal saline, n=20), GRg3 group (gavage of 20 mg/kg, 40 mg/kg and 80 mg/kg GRg3, n=20 for each dose) and Smecta mixture enema group (n=20). After 2 weeks, all rats were anesthetized with chloral hydrate (3 ml/kg) and approximately 5 ml of blood sample was collected from the abdominal aorta prior to sacrifice. The rectal tissues were taken and prepared for detection of Bax and NF-κB contents and HE staining to observe the pathological changes of the rectal tissues. After the blood samples were centrifuged, the supernatants were collected for ELISA to detect the serum levels of IL-2, IL-6, TNF-α and MPO in each group. Results In the GRg3 group, the serum inflammatory cytokines, serum MPO concentration and the concentration of Bax and NF-κB in the intestinal tissues of rats were decreased along with the increasing dose of GRg3. These parameters in the high-dose GRg3 group were significantly lower than those in the saline group (all P 0.05), suggesting that GRg3 exerted good therapeutic effect on acute radiation proctitis in rat models. Conclusions GRg3 can significantly reduce the concentration of inflammatory cytokines, Bax and NF-κB in the intestinal tissues of rat models with acute radiation proctitis, which is more efficacious than the normal saline. GRg3 can be used to treat acute radiation proctitis in rat models. Key words: Ginsenoside Rg3; Acute radiation proctitis; Rat
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来源期刊
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期刊介绍: The Chinese Journal of Radiation Oncology is a national academic journal sponsored by the Chinese Medical Association. It was founded in 1992 and the title was written by Chen Minzhang, the former Minister of Health. Its predecessor was the Chinese Journal of Radiation Oncology, which was founded in 1987. The journal is an authoritative journal in the field of radiation oncology in my country. It focuses on clinical tumor radiotherapy, tumor radiation physics, tumor radiation biology, and thermal therapy. Its main readers are middle and senior clinical doctors and scientific researchers. It is now a monthly journal with a large 16-page format and 80 pages of text. For many years, it has adhered to the principle of combining theory with practice and combining improvement with popularization. It now has columns such as monographs, head and neck tumors (monographs), chest tumors (monographs), abdominal tumors (monographs), physics, technology, biology (monographs), reviews, and investigations and research.
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