青萼素A通过影响IL-6在HCCLM3细胞中的表达调控STAT3信号通路

Q4 Immunology and Microbiology

中华微生物学和免疫学杂志 Pub Date : 2019-08-31 DOI:10.3760/CMA.J.ISSN.0254-5101.2019.08.008

Linlin Zhu, Zhixuan Xu, Mingming Zhang

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引用次数: 0

摘要

目的探讨青光素A (glaucocalyxin A, GLA)对HCCLM3细胞信号转导及转录激活因子3 (STAT3)信号通路表达的影响，进一步了解GLA在肿瘤转移中的作用。方法用不同浓度的GLA (0.1 ng/ml、1 ng/ml、10 ng/ml、100 ng/ml、1 000 ng/ml)刺激HCCLM3细胞48 h，分别用qRT-PCR和Western blot检测IL-6、STAT3/pSTAT3Tyr705和MMP9 mRNA和蛋白水平的表达。Transwell侵袭和迁移实验分析细胞的侵袭和迁移。ELISA法测定培养基中IL-6的浓度。结果0.1 ng/ml GLA刺激HCCLM3细胞后，IL-6在mRNA和蛋白水平的表达、STAT3Tyr705的磷酸化、HCCLM3细胞的迁移和侵袭能力均显著增强，而1 000 ng/ml GLA对其有抑制作用。此外，0.1 ng/ml GLA处理的HCCLM3细胞分泌更多的IL-6。结论GLA可能通过调节IL-6的表达和STAT3Tyr705的磷酸化来影响HCCLM3细胞的转移。这些调节作用与GLA浓度密切相关。关键词:青萼新A;白细胞介素- 6;信号转导和转录激活因子3;肝癌

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Glaucocalyxin A regulates STAT3 signaling pathway through influencing IL-6 expression in HCCLM3 cells

Objective To investigate the effects of glaucocalyxin A (GLA) on the expression of signal transduction and activator of transcription 3 (STAT3) signaling pathway in HCCLM3 cells for better understanding the role of GLA in tumor metastasis. Methods HCCLM3 cells were stimulated by different concentrations (0.1 ng/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml and 1 000 ng/ml) of GLA for 48 h. Expression of IL-6, STAT3/pSTAT3Tyr705 and MMP9 at mRNA and protein levels were detected by qRT-PCR and Western blot, respectively. Transwell invasion and migration assays were performed to analyze cell invasion and migration. ELISA was used to measure the concentrations of IL-6 in culture media. Results Expression of IL-6 at mRNA and protein levels, STAT3Tyr705 phosphorylation, and the migration and invasion of HCCLM3 cells were significantly enhanced after stimulation of HCCLM3 cells with 0.1 ng/ml of GLA, but inhibited by GLA at the concentration of 1 000 ng/ml. Moreover, more IL-6 was secreted out of the HCCLM3 cells treated with 0.1 ng/ml of GLA. Conclusions GLA might affect the metastasis of HCCLM3 cells through regulating the expression of IL-6 and the phosphorylation of STAT3Tyr705. These regulatory effects were closely related to the concentration of GLA. Key words: Glaucocalyxin A; Interleukin-6; Signal transduction and activator of transcription 3; Hepatocarcinoma

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来源期刊

中华微生物学和免疫学杂志 Immunology and Microbiology-Virology

CiteScore

0.50

自引率

0.00%

发文量

6906

期刊介绍： Chinese Journal of Microbiology and Immunology established in 1981. It is one of the series of journal sponsored by Chinese Medical Association. The aim of this journal is to spread and exchange the scientific achievements and practical experience in order to promote the development of medical microbiology and immunology. Its main contents comprise academic thesis, brief reports, reviews, summaries, news of meetings, book reviews and trends of home and abroad in this field. The distinguishing feature of the journal is to give the priority to the reports on the research of basic theory, and take account of the reports on clinical and practical skills.