阴道分泌物中阴道加德纳菌毒力因子的遗传研究

Q4 Medicine
S. Abdullah, A. Althahab, S. Jabuk
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引用次数: 0

摘要

背景:阴道炎是影响女性生殖系统的最常见的感染性疾病。普遍存在阴道感染,即细菌性阴道病。阴道加德纳菌是一种几乎总是与细菌性阴道病相关的非细菌属。目的:分离和鉴定阴道菌。以及对阴道溶酶、唾液酸酶、磷脂酶等毒力的诊断。材料与方法:采集100例女性阴道标本,经哥伦比亚血琼脂培养,分离阴道支原体并检测部分毒力因子。结果:调查结果显示12(12%)的样本检测阴道弧菌阳性。,分别为(7%、26%、13%、8%和2%)分离自20 ~ 50岁的人群。PCR结果显示,12株阴道弧菌分别有4株(33%)、9株(75%)和6株(50%)的vly、sld和pho基因阳性。结论:健康的阴道微生物群对维持阴道健康和预防感染具有重要意义。细菌性阴道病是指阴道菌群平衡失调,被阴道内的病原菌(如阴道杆菌)所取代。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genetic study of Gardnerella vaginalis virulence factors isolated from vaginal discharge
Background: Vaginitis is the most prevalent infectious condition affecting the female genital system. Prevalent vaginal infections, namely, bacterial vaginosis. Gardnerella vaginallis is one abacterial genus almost always associated with bacterial vaginosis. Objective: Isolation and identification of G. vaginalis. and Diagnosis some virulence such as Vaginolysin, Sialidase, Phospholipase. Materials and Methods: Collected 100 vaginal samples from women then cultures in Columbia blood agar to isolated G. vaginalis and genetic detection some virulence factors. Results: The findings revealed that 12 (12%) of the samples tested positive for G. vaginalis., including (7%, 26%, 13%, 8%, and 2% isolated from 20 to 50 years age, respectively. The results of PCR showed that out of 12 isolates only 4 (33%), 9 (75%) and, 6 (50%) of G. vaginalis isolates gave positive results to vly, sld and, pho gene respectively gave positive results from 12 G. vaginalis isolates. Conclusions: A healthy vaginal microbiota is considered to be significant for maintaining vaginal health and preventing infections. Bacterial vaginosis is the condition that occurs when the balance of the vaginal flora is dysregulated, and replaced by pathogenic bacteria in the vagina like G. vaginalis.
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来源期刊
CiteScore
0.90
自引率
0.00%
发文量
21
审稿时长
8 weeks
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