血清miR-187、miR-143在胆囊癌诊断中的临床意义

Q4 Medicine

中华肝胆外科杂志 Pub Date : 2020-02-28 DOI:10.3760/CMA.J.ISSN.1007-8118.2020.02.010

Yiming Chen, Yun-jie Wang, Deng-qiu Zhao, Delin Kong, Yong Wang

{"title":"血清miR-187、miR-143在胆囊癌诊断中的临床意义","authors":"Yiming Chen, Yun-jie Wang, Deng-qiu Zhao, Delin Kong, Yong Wang","doi":"10.3760/CMA.J.ISSN.1007-8118.2020.02.010","DOIUrl":null,"url":null,"abstract":"Objective \nTo investigate the clinical significance of serum miR-187 and miR-143 in the development and diagnosis of gallbladder cancer. \n \n \nMethods \n75 serum samples in patients with gallbladder cancer were selected as gallbladder cancer group. 75 serum samples in patients with gallbladder benign disease and 45 serum samples in healthy physical examinations at same period were selected as the benign gallbladder disease group and healthy control group. Quantitative RT-PCR was used to detect the serum miR-187 and miR-143 expression in each group, and the expression of those related with the clinicopathological factors, the proliferation and migration of gallbladder cancer cells, and the efficacy in diagnosis of gallbladder cancer was observed. \n \n \nResults \nThe serum miR-187 expression in gallbladder cancer group was significantly higher than that in benign gallbladder disease and healthy control; the serum expression of that in benign gallbladder disease was significantly higher than that in healthy control; after surgery , the expression of that was significantly lower than that before treatment (all P 0.05), and were significantly correlated with Nevin stage, TNM stage, differentiation and lymphatic metastasis (all P<0.05). Furthermore, it was confirmed that miR-187 promoted the proliferation and migration of gallbladder cancer cells in vitro, while miR-143 inhibited the proliferation and migration. In the diagnosis of gallbladder, the diagnostic efficacy of miR-187 and miR-143 was significantly better than that of CA199 and CA242 (both P<0.05). Combined detection could further improve the efficacy in diagnosis of gallbladder cancer. \n \n \nConclusions \nmiR-187 and miR-143 are involved in the development of gallbladder cancer. Combined detection of serum miR-187 and miR-143 in gallbladder cancer has a high diagnostic efficiency in the diagnosis of gallbladder cancer. \n \n \nKey words: \nGall bladder neoplasm; MiR-187; MiR-143; Diagnosis; Real-time -PCR","PeriodicalId":10021,"journal":{"name":"中华肝胆外科杂志","volume":"26 1","pages":"128-133"},"PeriodicalIF":0.0000,"publicationDate":"2020-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Clinical significance of serum miR-187 and miR-143 in the diagnosis of gallbladder cancer\",\"authors\":\"Yiming Chen, Yun-jie Wang, Deng-qiu Zhao, Delin Kong, Yong Wang\",\"doi\":\"10.3760/CMA.J.ISSN.1007-8118.2020.02.010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo investigate the clinical significance of serum miR-187 and miR-143 in the development and diagnosis of gallbladder cancer. \\n \\n \\nMethods \\n75 serum samples in patients with gallbladder cancer were selected as gallbladder cancer group. 75 serum samples in patients with gallbladder benign disease and 45 serum samples in healthy physical examinations at same period were selected as the benign gallbladder disease group and healthy control group. Quantitative RT-PCR was used to detect the serum miR-187 and miR-143 expression in each group, and the expression of those related with the clinicopathological factors, the proliferation and migration of gallbladder cancer cells, and the efficacy in diagnosis of gallbladder cancer was observed. \\n \\n \\nResults \\nThe serum miR-187 expression in gallbladder cancer group was significantly higher than that in benign gallbladder disease and healthy control; the serum expression of that in benign gallbladder disease was significantly higher than that in healthy control; after surgery , the expression of that was significantly lower than that before treatment (all P 0.05), and were significantly correlated with Nevin stage, TNM stage, differentiation and lymphatic metastasis (all P<0.05). Furthermore, it was confirmed that miR-187 promoted the proliferation and migration of gallbladder cancer cells in vitro, while miR-143 inhibited the proliferation and migration. In the diagnosis of gallbladder, the diagnostic efficacy of miR-187 and miR-143 was significantly better than that of CA199 and CA242 (both P<0.05). Combined detection could further improve the efficacy in diagnosis of gallbladder cancer. \\n \\n \\nConclusions \\nmiR-187 and miR-143 are involved in the development of gallbladder cancer. Combined detection of serum miR-187 and miR-143 in gallbladder cancer has a high diagnostic efficiency in the diagnosis of gallbladder cancer. \\n \\n \\nKey words: \\nGall bladder neoplasm; MiR-187; MiR-143; Diagnosis; Real-time -PCR\",\"PeriodicalId\":10021,\"journal\":{\"name\":\"中华肝胆外科杂志\",\"volume\":\"26 1\",\"pages\":\"128-133\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-02-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华肝胆外科杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1007-8118.2020.02.010\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华肝胆外科杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1007-8118.2020.02.010","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

摘要

目的探讨血清miR-187、miR-143在胆囊癌发生及诊断中的临床意义。方法选取75例胆囊癌患者血清标本作为胆囊癌组。选取胆囊良性疾病患者血清标本75份和同期健康体检血清标本45份作为胆囊良性疾病组和健康对照组。采用定量RT-PCR检测各组患者血清miR-187、miR-143的表达情况，并观察与临床病理因素、胆囊癌细胞增殖和迁移相关的miR-187、miR-143的表达情况以及对胆囊癌的诊断作用。结果胆囊癌组血清miR-187表达水平明显高于胆囊良性疾病组和健康对照组;良性胆囊疾病患者血清中表达量显著高于健康对照组;术后，其表达水平明显低于治疗前(均P<0.05)，且与Nevin分期、TNM分期、分化及淋巴转移相关(均P<0.05)。进一步证实，miR-187在体外促进了胆囊癌细胞的增殖和迁移，而miR-143抑制了胆囊癌细胞的增殖和迁移。在胆囊诊断中，miR-187、miR-143的诊断效能显著优于CA199、CA242 (P均<0.05)。联合检测可进一步提高胆囊癌的诊断效能。结论miR-187和miR-143参与了胆囊癌的发生发展。联合检测血清miR-187和miR-143对胆囊癌的诊断具有较高的诊断效率。关键词:胆囊肿瘤;mir - 187;mir - 143;诊断;实时聚合酶链反应

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Clinical significance of serum miR-187 and miR-143 in the diagnosis of gallbladder cancer

Objective To investigate the clinical significance of serum miR-187 and miR-143 in the development and diagnosis of gallbladder cancer. Methods 75 serum samples in patients with gallbladder cancer were selected as gallbladder cancer group. 75 serum samples in patients with gallbladder benign disease and 45 serum samples in healthy physical examinations at same period were selected as the benign gallbladder disease group and healthy control group. Quantitative RT-PCR was used to detect the serum miR-187 and miR-143 expression in each group, and the expression of those related with the clinicopathological factors, the proliferation and migration of gallbladder cancer cells, and the efficacy in diagnosis of gallbladder cancer was observed. Results The serum miR-187 expression in gallbladder cancer group was significantly higher than that in benign gallbladder disease and healthy control; the serum expression of that in benign gallbladder disease was significantly higher than that in healthy control; after surgery , the expression of that was significantly lower than that before treatment (all P 0.05), and were significantly correlated with Nevin stage, TNM stage, differentiation and lymphatic metastasis (all P<0.05). Furthermore, it was confirmed that miR-187 promoted the proliferation and migration of gallbladder cancer cells in vitro, while miR-143 inhibited the proliferation and migration. In the diagnosis of gallbladder, the diagnostic efficacy of miR-187 and miR-143 was significantly better than that of CA199 and CA242 (both P<0.05). Combined detection could further improve the efficacy in diagnosis of gallbladder cancer. Conclusions miR-187 and miR-143 are involved in the development of gallbladder cancer. Combined detection of serum miR-187 and miR-143 in gallbladder cancer has a high diagnostic efficiency in the diagnosis of gallbladder cancer. Key words: Gall bladder neoplasm; MiR-187; MiR-143; Diagnosis; Real-time -PCR

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

中华肝胆外科杂志 Medicine-Gastroenterology

CiteScore

0.20

自引率

0.00%

发文量

7101

期刊介绍： Chinese Journal of Hepatobiliary Surgery is an academic journal organized by the Chinese Medical Association and supervised by the China Association for Science and Technology, founded in 1995. The journal has the following columns: review, hot spotlight, academic thinking, thesis, experimental research, short thesis, case report, synthesis, etc. The journal has been recognized by Beida Journal (Chinese Journal of Humanities and Social Sciences). Chinese Journal of Hepatobiliary Surgery has been included in famous databases such as Peking University Journal (Chinese Journal of Humanities and Social Sciences), CSCD Source Journals of China Science Citation Database (with Extended Version) and so on, and it is one of the national key academic journals under the supervision of China Association for Science and Technology.