枣贻贝化学钻孔候选钙结合基因研究

IF 0.7 4区生物学 Q4 ZOOLOGY

Malacologia Pub Date : 2019-03-01 DOI:10.4002/040.062.0208

U. Sivka

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引用次数: 1

摘要

枣贻贝Lithophaga Lithophaga是一种化学蛀虫，它利用白质腺的分泌物在基质上钻孔。钙结合蛋白通常被认为是导致钙质底物化学溶解的原因。本研究的目的是研究5个候选基因——ANXB9、SCP、CALR、PLA2和V-ATPase在4种不同组织中的相对表达，并假设候选基因在苍白腺中的表达谱与其他基因不同。基因表达分析结果显示，CALR、PLA2和SCP在苍白腺中表达上调。其中两个候选基因(ANXB9和V-ATPase)在所有四种研究组织中具有相同的表达谱，表明它们不直接参与化学诱导机制。本研究结果揭示了枣贻贝化学钻孔机制的遗传背景。这组候选基因可用于枣贻贝和其他海洋或淡水钻孔生物的化学钻孔研究。

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Candidate Calcium-Binding Genes for Chemical Boring in the Date Mussel Lithophaga lithophaga

ABSTRACT The date mussel Lithophaga lithophaga is a chemical borer that uses pallial gland secretions to bore holes in the substrate. Calcium-binding proteins are generally considered to be responsible for the chemical dissolution of the calcareous substrate. The aim of this study was to investigate the relative expression of five candidate genes – ANXB9, SCP, CALR, PLA2 and V-ATPase – in four different tissues, with the hypothesis that the expression profile of the candidate genes in the pallial gland would differ from the rest. The results from gene expression analysis revealed that CALR, PLA2 and SCP were up-regulated in the pallial gland. Two of the candidate genes (ANXB9 and V-ATPase) had the same expression profile in all four investigated tissues, indicating that they are not directly involved in the chemical boring mechanism. The results of this study shed light on the genetic background of the chemical boring mechanism in date mussel. This set of candidate genes could be used in future research studies of chemical boring in the date mussel and other marine or freshwater borers.

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来源期刊

Malacologia 生物-动物学

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Malacologia publishes papers on all groups of the Mollusca. Malacologia specializes in publishing long papers and monographic treatments. Complete data are especially appreciated. Papers must be of interest to an international readership. Papers in systematics, ecology, population ecology, genetics, molecular genetics, evolution and phylogenetic treatments are especially welcomed. Also welcomed are letters to the editor involving papers published or issues of import to science of the day.