周期阈值在检测严重急性呼吸综合征冠状病毒患者病情及转归中的重要性

IF 0.1 Q4 MEDICINE, GENERAL & INTERNAL
B. Archana, K. Benachinmardi, SuraAnjanappa Lakshminarayana, R. Prakash, S. Sangeetha
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引用次数: 0

摘要

背景与目的:严重急性呼吸综合征冠状病毒2型(严重急性呼吸系统综合征冠状病毒冠状病毒2型)是2019年末出现的一种高传染性、高致病性的冠状病毒。实时逆转录聚合酶链式反应(RT-PCR)检测的循环阈值(Ct)值与病毒载量呈负相关,可以提供一种间接定量样本中病毒RNA拷贝数的方法,但临床上尚未报道。因此,本研究旨在将RT-PCR检测出的严重急性呼吸系统综合征冠状病毒2型阳性患者的Ct值与疾病严重程度、住院时间和死亡率进行比较。材料和方法:在班加罗尔的一家三级护理医院进行了为期6个月的回顾性研究。所有通过RT-PCR检测出严重急性呼吸系统综合征冠状病毒2型呈阳性并入住我院的患者均被纳入研究。收集患者的住院时间、年龄、合并症的存在、插管和死亡率的详细信息。结果:研究包括80名患者,48名(60%)男性和32名(40%)女性。研究人群的平均年龄为38.38岁。大多数患者的Ct值在25到30之间,占41.25%。Ct值较低的患者与住院时间增加显著相关,并且感染了一个以上的家庭成员,表明感染传播的可能性较高。死亡率与60岁以上的患者有显著相关性。解释和结论:该研究表明,严重急性呼吸系统综合征冠状病毒2型RT-PCR检测的Ct值与住院时间、传染性和死亡率之间可能存在关联。Ct值的提及以及阳性报告可能用于指导患者护理管理、感染控制和职业健康决策。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Importance of cycle threshold values in detection of severity and outcome in patients with severe acute respiratory syndrome coronavirus 2
Background and Objectives: Severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) is a highly transmissible and pathogenic coronavirus that emerged in late 2019. Cycle threshold (Ct) value of real-time reverse transcription polymerase chain reaction (RT-PCR) assay inversely correlated with viral load and can provide an indirect method of quantifying the number of copies of viral RNA in the sample is not reported clinically. Hence, this study was undertaken to compare the Ct values of patients tested positive for SARS CoV-2 by RT-PCR with severity of illness, duration of hospital stay, and mortality. Materials and Methods: A retrospective study was conducted over a period of 6 months in a tertiary care hospital in Bangalore. All patients tested positive for SARS CoV-2 by RT-PCR and admitted in our hospital were included in the study. Details of the patients on the duration of hospital stay, age, presence of comorbidities, intubation, and mortality were collected. Results: The study comprised of 80 patients, 48 (60%) males and 32 (40%) females. The mean age of the study population was 38.38 years. Majority of patients 41.25% had Ct value between 25 and 30. Patients with lower Ct values were significant associated with increased duration of hospital stay and infected more than one person in family indicating higher probability of transmission of infection. Mortality showed significant association with patients of more than 60 years' age. Interpretation and Conclusions: The study shows possible association between Ct values of SARS-CoV-2 RT-PCR assay with the duration of hospitalization, infectivity, and mortality. Mention of Ct value along with the positive report could potentially be used to guide patient care management, infection control, and occupational health decisions.
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来源期刊
Journal of the Scientific Society
Journal of the Scientific Society MEDICINE, GENERAL & INTERNAL-
自引率
33.30%
发文量
19
审稿时长
36 weeks
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