云芝和硫花总酚、总黄酮、金属含量及抗氧化活性的测定

IF 0.6 4区农林科学 Q4 HORTICULTURE

Acta Scientiarum Polonorum Hortorum Cultus Pub Date : 2022-10-28 DOI:10.24326/asphc.2022.5.4

Sanem Bulam, Mertcan Karadeniz, T. Bakır, S. Ünal

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引用次数: 3

摘要

采用电感耦合等离子体发射光谱法和分光光度法，对从卡斯塔莫努地区采集的云芝（Trametes versicolor）和硫花（Laetiporus sulfureus）的抗氧化活性、金属含量、总酚类和黄酮类化合物进行了分析。蘑菇提取物的抗氧化活性以DPPH自由基抑制百分比和IC50值表示。对L.sulfuureus的抑制率在15.83%至61.03%之间，IC50值为5.33mg/mL，对T.versicolor的抑制率为7.27%至20.47%，IC50为18.10mg/mL。以没食子酸当量表示的甲醇提取物中酚类的含量变化范围分别为77.41±1.10和272,70±2.26 mg/g。此外，以槲皮素当量表示的甲醇提取物中黄酮类化合物的含量，云芝和硫磺乳杆菌分别在13.82±0.21和44.29±0.54mg/mg之间变化。结果证实了该蘑菇的总酚含量和抗氧化活性之间存在显著的线性相关性。此外，通过ICP-OES对所有样品进行分析，以获得Co、Cu、Cd、Pb、Ni、Cr、Na、Ca、Al、Fe、Zn、Ba、P、Mg、As、Mn和B的浓度。蘑菇的最大和最小金属含量分别为Mg/kg（1.88-2.09）、Mg（4.59-6.77）、P（17.11-24.52）、Ca（0.49-23.91）和Fe（0.49-3.84），蘑菇中Cr（8.23-35.27）、Ni（41.51-180.99）、Cu（36.36-123.93）的最高和最低微量金属含量分别为µg/kg。

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Assessment of total phenolic, total flavonoid, metal contents and antioxidant activities of Trametes versicolor and Laetiporus sulphureus

Antioxidant activities, metal contents, total phenolics and flavonoids were analyzed for Trametes versicolor and Laetiporus sulphureus collected from Kastamonu region by using ICP-OES and spectrophotometric methods. Antioxidant activity of mushroom extracts were expressed as percentage of DPPH radicals inhibition and IC50 values. Percentage of inhibition ranged from 15.83 to 61.03% and IC50 value was found 5.33 mg/mL for L. sulphureus and % inhibition ranged from 7.27 to 20.47% and IC50 value was found 18.10 mg/mL for T. versicolor. The content of phenols in methanolic extracts expressed in gallic acid equivalents varied between 77.41±1.10 and 272,70±2.26 mg/g for T. versicolor and L. sulphureus respectively. Also the content of flavonoids in methanolic extracts expressed in quercetin equivalents varied between 13.82±0.21 and 44.29±0.54 mg/mg for T. versicolor and L. sulphureus, respectively. As a result the significant linear correlation was corroborated between the values for the total phenolic content and antioxidant activity of this mushroom. In addition, all samples were analyzed by ICP-OES to obtain the concentration of Co, Cu, Cd, Pb, Ni, Cr, Na, Ca, Al, Fe, Zn, Ba, P, Mg, As, Mn and B. While maximum and minimum metal contents of mushrooms were found as mg/kg for Na (1.88-2.09), Mg (4.59-6.77), P (17.11-24.52), Ca (0.49-23.91) and Fe (0.49-3.84), the maximum and minimum trace metal contents of mushrooms were found as µg/kg for Cr (8.23-35.27), Ni (41.51-180.99), Cu (36.36-123.93).

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来源期刊

Acta Scientiarum Polonorum Hortorum Cultus 农林科学-园艺

CiteScore

1.30

自引率

14.30%

发文量

审稿时长

4-8 weeks

期刊介绍： In Acta Scientiarum Polonorum Hortorum Cultus we publish original research papers and review articles containing new and significant information on broad aspects of horticulture and related disciplines. The papers are published in English only, in six issues yearly.