Greenness Evaluation and Simultaneous High-Performance Thin-Layer Chromatography Method for Determination of Powder Mixture and Ayurvedic Formulation

Quality is the essential aspect of pharmaceuticals for both synthetic and herbal products. The quality assessment of the herbals is the most challenging task. In this study, analytical method is developed for simultaneous estimation of the three different standards. The separation method known as high-performance thin-layer chromatography (HPTLC) is very adaptable and economical. The method developed on HPTLC system where in the stationary phase used is tlc silica gel G F254 with aluminum backing and the mobile phase finalized was ethyl acetate: hexane: formic acid (5:5:1) v/v/v. The developed method was then validated in accordance with International Conference on Harmonization and United State Pharmacopeia guidelines. After validation, the developed method was found to be easy, simple, precise and specific. The greenness of the method was evaluated using the AGREE scale.