内皮抑素和血管抑素在翼状胬肉中的表达及临床意义

中国基层医药 Pub Date : 2020-04-01 DOI:10.3760/CMA.J.ISSN.1008-6706.2020.07.006

Jinrong Zhao, Weiwei Chen, Zhongping Jin, Zheng Zhang

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引用次数: 0

摘要

目的探讨内皮抑素（ES）和血管抑素（AS）在翼状胬肉中的表达及其临床意义。方法2016年1月至2018年12月，从义乌市中医院眼科手术中选取60例（60眼）翼状胬肉组织和60例（6 0眼）正常人结膜组织，采用HE染色观察翼状胬肉和正常结膜组织的形态学变化。采用免疫印迹法检测翼状胬肉组和对照组组织中ES和AS蛋白水平。结果HE染色显示正常球结膜组织间质层为结缔组织，上皮层为柱状上皮；翼状胬肉基底层有大量新生血管、成纤维细胞、胶原纤维和炎症细胞浸润于血管周围；上皮细胞呈现不同程度的增生。翼状胬肉组织中ES和AS的蛋白水平[（0.35±0.12），（0.62±0.17）]高于对照组[（0.13±0.08），（0.16±0.09）]（t=11.816，18.524，P=0.0000,0.000）（t=17.073，12.323，P=0.000，0.000）。翼状胬肉ES和AS呈正相关（r=0.571，P=0.000）。结论翼状胬肉组织中ES和AS水平升高，ES和AS可能参与翼状胬肉的发生和复发。关键词：翼状胬肉；抑制素；血管他汀类药物；内皮细胞；重复

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Expression and clinical significance of endostatin and angiostatin in pterygium

Objective To investigate the expression and clinical significance of endostatin (ES) and angiostatin (AS) in pterygium. Methods From January 2016 to December 2018, 60 cases (60 eyes) of pterygium tissue and 60 cases (60 eyes) of normal human conjunctival tissue were selected from the eye surgery of Traditional Chinese Medicine Hospital of Yiwu.HE staining was used to observe the morphological changes of pterygium and normal conjunctival tissue.Western blot was used to measure ES and AS protein levels in the tissues of pterygium group and control group. Results HE staining showed that in the normal bulbar conjunctival tissue, the stromal layer was connective tissue and the epithelial layer was columnar epithelium; in the pterygium, the basal layer had a large number of new blood vessels, fibroblasts, collagen fibers, and inflammatory cells infiltrated around the blood vessels; the epithelium showed different degrees of hyperplasia.The protein levels of ES and AS in pterygium tissues[(0.35±0.12), (0.62±0.17)] were higher than those in the control group [(0.13±0.08), (0.16±0.09)](t=11.816, 18.524, P=0.000, 0.000). The protein levels of ES and AS in the pterygium tissues of the recurrent group [(0.63±0.15), (0.87±0.21)] were higher than those in the initial group [(0.22±0.11), (0.45±0.16)](t=17.073, 12.323, P=0.000, 0.000). There was positive correlation between ES and AS in pterygium (r=0.571, P=0.000). Conclusion The levels of ES and AS in pterygium tissue are increased, and ES and AS may be involved in the occurrence and recurrence of pterygium. Key words: Pterygium; Inhibins; Angiostatins; Endothelial cells; Recurrence

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来源期刊

中国基层医药

CiteScore

0.10

自引率

0.00%

发文量

32251

期刊介绍： Since its inception, the journal "Chinese Primary Medicine" has adhered to the development strategy of "based in China, serving the grassroots, and facing the world" as its publishing concept, reporting a large amount of the latest medical information at home and abroad, prospering the academic field of primary medicine, and is praised by readers as a medical encyclopedia that updates knowledge. It is a core journal in China's medical and health field, and its influence index (CI) ranks Q2 in China's academic journals in 2022. It was included in the American Chemical Abstracts in 2008, the World Health Organization Western Pacific Regional Medical Index (WPRIM) in 2009, and the Japan Science and Technology Agency Database (JST) and Scopus Database in 2018, and was included in the Wanfang Data-China Digital Journal Group and the China Academic Journal Comprehensive Evaluation Database.