非致病性和致病性农杆菌间基因的偶联转移频率

IF 1.2 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

National Academy Science Letters Pub Date : 2023-05-09 DOI:10.1007/s40009-023-01255-6

Aditi Sharma, A. K. Gupta

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引用次数: 0

摘要

本研究对UHFBA-218对植物致病性农杆菌的生物药效、转偶联形成及毒力基因获得进行了较详细的研究。将拮抗农杆菌(UHFBA-218和K84)和致病性农杆菌按1:1细胞比接种于番茄茎上。共发现异共轭物240例;UHFBA-218接种的36株和k84接种的55株均表现出农业蛋白活性并对其免疫。UHFBA-218的4个转偶联物与Vir D2和ipt基因均有扩增。总体结果表明，菌株UHFBA-218是抑制胆汁形成的良好候选者，因为它不获得毒力基因。

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Frequency of Conjugal Transfer of Genes Between Non-pathogenic and Pathogenic Agrobacteria

In the present study, a detailed investigation was done on bioefficacy, transconjugant formation and virulence gene acquisition of UHFBA-218 against pathogenic agrobacteria in planta. Antagonistic (UHFBA-218 and K84) and pathogenic agrobacteria were co-inoculated on tomato stems at a 1:1 cell ratio. A total of 240 transconjugants were recovered; 36 from UHFBA-218 and 55 from K84-inoculated plants showed an agrocin activity and were immune to it. Four transconjugants of UHFBA-218 showed amplification with Vir D2 and ipt genes. The overall results suggest that strain UHFBA-218 is a good candidate for suppressing gall formation as it does not acquire the virulence genes.

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来源期刊

National Academy Science Letters 综合性期刊-综合性期刊

CiteScore

2.20

自引率

0.00%

发文量

审稿时长

12 months

期刊介绍： The National Academy Science Letters is published by the National Academy of Sciences, India, since 1978. The publication of this unique journal was started with a view to give quick and wide publicity to the innovations in all fields of science