加利福尼亚对虾外骨骼中几丁质和壳聚糖的物理化学研究

IF 0.5 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
K. G. Martinez-Robinson, Alfredo Martínez-Inzunza, Sarai Rochín-Wong, Rosalva Josefina Rodríguez Córdova, S. R. Vasquez-Garcia, Daniel Fernández-Quiroz
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引用次数: 0

摘要

这项工作描述了从加利福尼亚湾采集的褐虾外骨骼(Farfentepenaeus californiensis)中获得几丁质和壳聚糖。甲壳素分离过程是通过连续处理进行的,包括脱矿和脱蛋白。另一方面,通过碱水解制备壳聚糖,并使用膜过滤器系列对产物进行纯化,以使大分子链的大小均匀化。根据该方法,甲壳素和壳聚糖的产率分别约为34%和20%(干重)。通过红外光谱和热重分析对材料进行了表征。此外,通过近端测试、粘均分子量和脱乙酰度对壳聚糖样品进行了分析。该产品的化学结构和物理化学性质与文献中报道的从类似来源分离的生物聚合物相当。发现壳聚糖的乙酰化程度为15.87%(通过1H-NMR分析)并且Mv=170kDa。结果表明,来自科尔特斯海海岸的壳聚糖废物可以被认为是制备壳聚糖的一个有前途的来源,并可用于该地区的多种应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Estudio Fisicoquímico de Quitina y Quitosana obtenidas a partir del exoesqueleto de camarón café (Farfantepenaeus californiensis)
This work describes the obtention of chitin and chitosan from the brown shrimp exoskeleton (Farfentepenaeus californiensis), which is collected from the Gulf of California. The chitin isolation process was carried out by a consecutive treatment that includes demineralization and deproteinization. On the other hand, the preparation of chitosan was conducted through alkaline hydrolysis, and the product was purified by using a membrane filters series to homogenize the size of the macromolecular chains. According to the methodology, the yield of chitin and chitosan was around 34 % and 20 % (dry weight), respectively. The materials were characterized by FTIR spectroscopy and thermogravimetric analysis. Also, the chitosan sample was analyzed by proximal test, viscometric-average molecular weight, and deacetylation degree. The products exhibited chemical structure and physicochemical properties comparable to biopolymers isolated from similar sources reported in the literature. Chitosan was found to have a degree of acetylation of 15.87 % (by 1H-NMR analysis) and Mv=170 kDa. The results suggest that the chitinous waste from the Sea of Cortes coast can be considered a promising source for the preparation of chitosan and used for several applications in the region.
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来源期刊
Biotecnia
Biotecnia BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
自引率
33.30%
发文量
39
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