{"title":"结直肠癌caspase 3、8、9基因及ADIPOR1 (rs2275738)多态性的分子检测","authors":"Zainab Nizar Jawad","doi":"10.1007/s13204-023-02879-2","DOIUrl":null,"url":null,"abstract":"<div><p>The main type of cancer-related world casualties is colorectal cancer (CRC). Colorectal cancer occurs due to many important factors, including genes. These genes are programmed death genes such as caspase 3, 8, 9 and variants in receptor of adiponectin 1 (ADIPOR1) that are related to ‘CRC’ risk. A study was conducted on the high and low gene expression of caspase 3, 8, and 9 genes and their association with the risk of rectal cancer, as well as the association of genotypes in adiponectin receptor 1 (ADIPOR1) to indicate the extent to which these genotypes are related to colorectal cancer compared with the control group. This study was conducted from 2021 to 2022 on 100 patients with colorectal cancer after being medically diagnosed and compared them with 100 healthy individuals. Overall mRNA for all blood samples were taken out and cDNA was produced, use of SYBR Green mix of PCR master and actual gene expression method, quantifying the transcript level of target genes. The polymorphism of the genotypes of ADIPOR1 (rs2275738) was studied using the PCR–RFLP method. Experiments revealed the mRNA expressions of caspases 9 and 3 in patients with lower CRC compared to healthy control patients and controls. Nonetheless, ADIPOR1 (rs2275738) “CC + CT” genotype in comparison to the “TT” genotype was more repeatedly found in CRC cases than in controls. Smoking, body mass index, and family history of colorectal cancer were higher in the CRC group when compared with the control group.</p></div>","PeriodicalId":471,"journal":{"name":"Applied Nanoscience","volume":"13 8","pages":"5365 - 5368"},"PeriodicalIF":3.6740,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular detection of caspase 3, 8, 9 genes and ADIPOR1 (rs2275738) polymorphism in colorectal cancer\",\"authors\":\"Zainab Nizar Jawad\",\"doi\":\"10.1007/s13204-023-02879-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The main type of cancer-related world casualties is colorectal cancer (CRC). Colorectal cancer occurs due to many important factors, including genes. These genes are programmed death genes such as caspase 3, 8, 9 and variants in receptor of adiponectin 1 (ADIPOR1) that are related to ‘CRC’ risk. A study was conducted on the high and low gene expression of caspase 3, 8, and 9 genes and their association with the risk of rectal cancer, as well as the association of genotypes in adiponectin receptor 1 (ADIPOR1) to indicate the extent to which these genotypes are related to colorectal cancer compared with the control group. This study was conducted from 2021 to 2022 on 100 patients with colorectal cancer after being medically diagnosed and compared them with 100 healthy individuals. Overall mRNA for all blood samples were taken out and cDNA was produced, use of SYBR Green mix of PCR master and actual gene expression method, quantifying the transcript level of target genes. The polymorphism of the genotypes of ADIPOR1 (rs2275738) was studied using the PCR–RFLP method. Experiments revealed the mRNA expressions of caspases 9 and 3 in patients with lower CRC compared to healthy control patients and controls. Nonetheless, ADIPOR1 (rs2275738) “CC + CT” genotype in comparison to the “TT” genotype was more repeatedly found in CRC cases than in controls. Smoking, body mass index, and family history of colorectal cancer were higher in the CRC group when compared with the control group.</p></div>\",\"PeriodicalId\":471,\"journal\":{\"name\":\"Applied Nanoscience\",\"volume\":\"13 8\",\"pages\":\"5365 - 5368\"},\"PeriodicalIF\":3.6740,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Nanoscience\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13204-023-02879-2\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Engineering\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Nanoscience","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s13204-023-02879-2","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Engineering","Score":null,"Total":0}
Molecular detection of caspase 3, 8, 9 genes and ADIPOR1 (rs2275738) polymorphism in colorectal cancer
The main type of cancer-related world casualties is colorectal cancer (CRC). Colorectal cancer occurs due to many important factors, including genes. These genes are programmed death genes such as caspase 3, 8, 9 and variants in receptor of adiponectin 1 (ADIPOR1) that are related to ‘CRC’ risk. A study was conducted on the high and low gene expression of caspase 3, 8, and 9 genes and their association with the risk of rectal cancer, as well as the association of genotypes in adiponectin receptor 1 (ADIPOR1) to indicate the extent to which these genotypes are related to colorectal cancer compared with the control group. This study was conducted from 2021 to 2022 on 100 patients with colorectal cancer after being medically diagnosed and compared them with 100 healthy individuals. Overall mRNA for all blood samples were taken out and cDNA was produced, use of SYBR Green mix of PCR master and actual gene expression method, quantifying the transcript level of target genes. The polymorphism of the genotypes of ADIPOR1 (rs2275738) was studied using the PCR–RFLP method. Experiments revealed the mRNA expressions of caspases 9 and 3 in patients with lower CRC compared to healthy control patients and controls. Nonetheless, ADIPOR1 (rs2275738) “CC + CT” genotype in comparison to the “TT” genotype was more repeatedly found in CRC cases than in controls. Smoking, body mass index, and family history of colorectal cancer were higher in the CRC group when compared with the control group.
期刊介绍：
Applied Nanoscience is a hybrid journal that publishes original articles about state of the art nanoscience and the application of emerging nanotechnologies to areas fundamental to building technologically advanced and sustainable civilization, including areas as diverse as water science, advanced materials, energy, electronics, environmental science and medicine. The journal accepts original and review articles as well as book reviews for publication. All the manuscripts are single-blind peer-reviewed for scientific quality and acceptance.
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