The effects of capparis spinosa ethanol extracts on humoral immune responses in mice treated with aflatoxin B1

The results of this investigation had demonstrated the production of aflatoxins from Aspergillus flavus and study effects on humoral immune by measuring the concentrations of immunoglobulins IgA, IgG, IgM, IgE and measurement of concentrations of the complements C3, C4 in mice with treated of Aflatoxin B1. The results of qualities analysis HPLC showed the ability of isolation of Aspergillus flavus from chips, macroni, red sun flower, shamia, hazelnut, corn and peanut and isolate from fish to produce aflatoxin with different rate. The isolation of Aspergillus flavus which isolated from peanuts recoded the highest rate of production of aflatoxin B1 which it reached to (213760.28) ppm. 40 male mice were used experimentation they divided into eight groups. Three groups of mice were gave aflatoxin B1 56.3 ppm and after 2 hours treated by ethanolic extracts crude phenol and alkaloid in 250 mg/ ml while the fourth group was given aflatoxin B1 only, while the fifth, sixth and seventh groups were treated with ethanolic extracts only and the eight group was given control groups PBS. The results of study showed a significant increase in concentrations immunoglobulins IgA, IgG, IgM in the mice that dosage of extracts crude after gave aflatoxin B1 56.3 ppm it reached the highest concentration of IgA, IgG, IgM (139. 06, 3223.80, 339.66) mg/ 100 ml respectively compared with positive control that gave aflatoxin only it reached IgA, IgG, IgM (20. 23, 108.66, 25.30) mg/ 100 ml respectively. The results of the study also recoded a significant increase in the IgE concentration of 22 IU in mice that gave the crude extract after giving aflatoxin B1 with concentrations of 56.3 ppm compared to positive control of 2.83 IU. The results of the study showed a significant increase in concentration of complements C3, C4 in mice that dosage ethanolic extracts crude phenols and alkaloid after two hours gave aflatoxin B1. The highest concentration of C3, C4 (279.33, 132.20) mg/ 100 ml respectively in mice that gave crude extract after given aflatoxin B1 in compared with positive control that gave aflatoxin B1 only it reached concentrations C3, C4 (12, 17.50) mg/ 100 ml respectively. We concluded of that caper plant has immune stimulatory effects in mice.