过氧化氢酶基因在氨环境下的表达谱

M. Nasr, A. Elkamel, M. Youssef, W. Emeish
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引用次数: 0

摘要

氨是限制水生生物生长和生存的最有害的水质参数之一。因此,鱼类必须通过改变受基因表达调控的生理过程来适应这种压力。本研究的目的是研究抗氧化相关基因过氧化氢酶(CAT)在鲤鱼(Cyprinus carpio)鱼种中暴露于0.7mg/l的水中联合氨(UIA)后的表达谱。在四个时间点(暴露后12小时、2天、4天和7天)测量肝脏、鳃和脑组织中的相对基因表达。CAT基因在肝脏和大脑中的表达水平在氨暴露7天后分别达到13.3和5.2倍的峰值,但在鳃中仅在2天后上调(2.7倍),在其他时间点下调。这项研究证明,暴露于氨会影响鲤鱼的抗氧化状态,CAT基因表达水平的改变表明了这一点
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression profiles of catalase gene in common carp exposed to ammonia
Ammonia is one of the most harmful water quality parameters restricting the growth and survival of aquatic living organisms. As a result, fish must adapt to this stressor by modifying physiological processes that are governed by gene expression regulation. The aim of the present study was to investigate the expression profiles of antioxidant related gene, catalase ( CAT ) in common carp ( Cyprinus carpio ) fingerlings after exposure to 0.7 mg/l of unionized ammonia (UIA) in water. The relative gene expression was measured in liver, gills, and brain tissues at four time points (12 h, 2 d, 4 d, and 7 d post exposure). The expression level of CAT gene in the liver and brain peaked after 7 d of ammonia exposure by 13.3 and 5.2-folds, respectively, but in gills it upregulated only after 2 d (2.7-folds) and downregulated at the other time points. This study proved that exposure to ammonia affects the antioxidant status of common carp as indicated by the altered levels of expression of CAT gene
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