Methodological Consideration for Endothelial Function Assessment based on Bilateral Differences

Examining the characteristics and responses of an individual’s vascular system is important for improving the early detection, prevention, and treatment of cardiovascular disease. For example, applanation tonometry is a common means of assessing arterial stiffness, with precise standards established for measurement [1]. Previous studies have examined potential differences in arterial stiffness between arms [2–5], with conflicting findings. Further, while the assessment of arterial stiffness can provide a snapshot of vascular health at rest, information on dynamic vascular function can be determined by assessing the change in Pulse Wave Velocity (PWV) in response to a condition of reactive hyperemia [6–16].