色谱分离中分配系数温度依赖性的热力学分析方法

Q4 Engineering

Japan Journal of Food Engineering Pub Date : 2019-09-15 DOI:10.11301/jsfe.19550t

João M. S. Cardoso, N. Yoshimoto, Shuichi Yamamoto

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引用次数: 0

摘要

色谱分离的热力学分析不仅对分离的优化，而且对开发新的分离介质和分离工艺具有重要意义。本研究采用范霍夫图和等温滴定量热计(ITC)分析了乙醇-水流动相聚合物基色谱法分离两种模型组分(多酚)时的温度依赖性。等温洗脱得到的分布系数K既用于范霍夫图，也用于ITC测量中吸附量的计算。两种方法得到的焓值相似。由于ITC不能预测分离行为，因此建议使用色谱实验作为分析分离温度依赖性的补充方法。

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Methods for Thermodynamic Analysis of Temperature Dependence of Distribution Coefficients in Chromatography Separations

Thermodynamic analysis of chromatography separation is important not only for optimization of separation but also development of new separation media and processes. In this study temperature dependence in the separation of two model components (polyphenols) by polymer-based chromatography with ethanol-water mobile phase was analyzed by van’t Hoff plots and isothermal titration calorimeter (ITC). The distribution coef ficient K obtained by the isocratic elution of chromatography was employed both for van’t Hoff plots and for the calculation of adsorbed amount in the ITC measurements. Enthalpy values obtained by both methods were similar. As ITC is not able to predict separation behavior, it is recommended to use chromatography experiments as a complimentary method for analyzing the temperature dependence of separation.

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来源期刊

Japan Journal of Food Engineering Engineering-Industrial and Manufacturing Engineering

CiteScore

0.50

自引率

0.00%

发文量

期刊介绍： The Japan Society for Food Engineering (the Society) publishes "Japan Journal of Food Engineering (the Journal)" to convey and disseminate information regarding food engineering and related areas to all members of the Society as an important part of its activities. The Journal is published with an aim of gaining wide recognition as a periodical pertaining to food engineering and related areas.