使用膜过滤从墨西哥锡那罗亚culiacan当地超市的生鸡肉样品中回收弯曲杆菌

IF 0.5 Q4 BIOLOGY
J. Soto-Beltrán, B. Quiñones, A. Ibarra-Rodríguez, B. A. Amézquita-López
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引用次数: 0

摘要

空肠弯曲杆菌和大肠弯曲杆菌是全世界人类肠胃炎的主因,食用生的和未煮熟的家禽会导致人类感染。本研究检查了从墨西哥锡那罗亚州库利亚坎市当地市场购买的零售鸡尸体中是否存在空肠杆菌和大肠杆菌。为了提高弯曲杆菌的回收率,将生鸡胴体的包装液在微气条件下富集过夜后进行膜过滤。通过lpxA基因的PCR扩增进一步确定了空肠杆菌和大肠杆菌的存在。从63.6%(7/11)的零售市场购买的鸡尸体中检出56.7%(17/30)的推测弯曲杆菌菌落。基因分型检测到C。33.3%(10/30)为空肠菌。本研究首次在墨西哥西北部的生鸡肉中发现了空肠梭菌,为零售肉类中弯曲杆菌的监测提供了有价值的信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Uso de filtración por membrana para la recuperación de Campylobacter de muestras de pollo crudo provenientes de supermercados locales en Culiacán, Sinaloa, México
Campylobacter  jejuni  and  Campylobacter coli  are responsible for human gastroenteritis worldwide, and the consumption of raw and undercooked poultry contributes to human infections. The present study examined the presence of  C. jejuni  and  C. coli  from retail chicken carcasses, purchased from local markets in the Culiacan Municipality in Sinaloa, Mexico.  To improve the recovery of  Campylobacter , the package liquid from the raw chicken carcasses was subjected to membrane filtration after an overnight enrichment under microaerophilic conditions.  The presence of  C. jejuni  and  C. coli  was further determined by PCR amplification of the  lpxA  gene.  Presumptive  Campylobacter  colonies were recovered from 56.7% (17/30) of the chicken carcasses purchased from 63.6% (7/11) of the retail markets examined.  Genotyping assays detected  C . jejuni  in 33.3% (10/30) of the carcasses examined.  This study identified for the first time  C. jejuni  in raw chicken carcasses in Northwestern Mexico and provided valuable information for monitoring  Campylobacter in retail meats.
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