香蕉品种Kachkal对Sigatoka感染防御反应基因的表达分析

IF 1 4区生物学 Q3 PLANT SCIENCES

Indian Journal of Genetics and Plant Breeding Pub Date : 2022-04-25 DOI:10.31742/ijgpb.82.2.11

Sudarshana Borah, Banashree Saikia, D. Bora, P. Bhorali

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引用次数: 0

摘要

香蕉叶斑病是香蕉的一种重要的经济病害，在世界范围内造成巨大的产量损失。本研究分析了抗性香蕉品种Kachkal与Sigatoka病菌不相容互作的防御相关基因表达。研究了侵染伪麻孢子菌侵染后部分防御相关基因表达的初始变化。半定量RT-PCR研究表明，这些基因在侵染前在寄主植物中表达水平基本不变，而侵染后部分重要的防御反应基因PR4、渗透素、LTP、UGPase和JAR1的表达水平均出现上调。该研究结果将有助于设计香蕉病遗传控制的精确育种策略。

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Expression analysis of defense response genes upon Sigatoka infection in Musa paradisiaca cultivar Kachkal

The Sigatoka disease is an economically important disease of banana as it causes tremendous yield losses all over the world. In the present study, the defense related gene expression implicated in an incompatible interaction between a resistant banana cultivar Kachkal and the Sigatoka pathogen was analyzed. The initial changes in the expression of some selected defense related genes after infection by the invading pathogen Pseudocercospora musae were studied. Semi-quantitative RT-PCR studies indicated a basal level of expression of the selected genes prior to infection in the host plant, while upregulation of some of the important defense response genes coding for PR4, osmotin, LTP, UGPase and JAR1 was observed after the infection by P. musae. The findings of the study would be useful in designing a precise breeding strategy for genetic control of the disease in banana.

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来源期刊

Indian Journal of Genetics and Plant Breeding PLANT SCIENCES-

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.