褪黑激素受体1A (MTNR1A)基因多态性与水牛生育力季节性抑制的关系

IF 0.2 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Buffalo Bulletin Pub Date : 2022-12-30 DOI:10.56825/bufbu.2022.4143569

Muddarangaiah Madyavenkatapura Rangashamaiah, S. Ghuman, R. Cheema

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引用次数: 0

摘要

褪黑激素受体1A (MTNR1A)基因多态性及其与生殖周期的关系(cyclic, n=51;对夏季非循环期(n=50)和初产犊年龄(3.5岁，n=60)的水牛进行了血液采样。采用MTNR1A基因外显子ii对应的824 bp(引物对1)、856 bp(引物对2)和268 bp(引物对3)3条引物对基因组DNA进行聚合酶链反应(PCR)。对引物1和引物2的PCR产物进行限制性内切片段长度多态性分析。用限制性内切酶MnII、RsaI或Eco3II对引物1的PCR产物进行酶切，得到的总限制性内切位点分别为14、4和5个。根据不同的酶切位点，MnII在水牛中发现6个基因型为MM-1 ~ MM-6, RsaI在水牛中发现4个基因型为RR-1 ~ RR-4, Eco3II在水牛中发现4个基因型为EE-1 ~ EE-4。用MnII、RsaI或Eco3II酶切引物对2的PCR产物，分别产生13、5、6个限制性位点。根据不同的酶切位点，MnII检测到的6个基因型为mm-1 ~ mm-6, RsaI检测到的5个基因型为rr-1 ~ rr-5, Eco3II检测到的3个基因型为ee-1 ~ ee-3。对引物3单链构象多态性分析显示，MTNR1A基因在夏季表现为MM-3、MM-4、r- 2、EE-3、mm-2、MM-4、rr-3、EE-3、CC和DD基因型，而在非循环期表现为mm-2、MM-6、EE-4、MM-3、EE-3、AA、BB和AB基因型。然而，在首次产犊的年龄方面，没有记录到这种差异。综上所述，MTNR1A基因的多态基因型可以作为鉴定夏季繁殖潜力较高水牛的遗传标记。

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Association of Melatonin receptor 1A (MTNR1A) gene polymorphism with seasonal suppression of fertility in buffaloes

The polymorphism in Melatonin receptor 1A (MTNR1A) gene and its association with reproductive cyclicity (cyclic, n=51; non-cyclic, n=50) during summer season and age at first calving (<3.5 years, n=38; >3.5 years, n=60) was investigated in buffaloes subjected to blood sampling. Polymerase chain reaction (PCR) of genomic DNA was carried out using three primers viz. 824 bp (primer pair 1), 856 bp (primer pair 2) and 268 bp (primer pair 3) corresponding to exon-II of MTNR1A gene. Restriction fragment length polymorphism was performed using PCR products of primer pair 1 and 2. The digestion of PCR product of primer pair 1 with restriction enzymes viz. MnII, RsaI or Eco3II generated total restriction sites respectively as 14, 4 and 5. Based upon differential restriction sites, six genotypes revealed in buffaloes using MnII were MM-1 to MM-6, four genotypes using RsaI were RR-1 to RR-4, and four genotypes using Eco3II were EE-1 to EE-4. The digestion of PCR product of primer pair 2 with MnII, RsaI or Eco3II generated restriction sites respectively as 13, 5 and 6. Based upon differential restriction sites, six genotypes revealed using MnII were mm-1 to mm-6, five genotypes using RsaI were rr-1to rr-5, and three genotypes using Eco3II were ee-1 to ee-3. For primer pair 3, single stranded conformational polymorphism analysis exhibited 5 genotypes viz. AA, BB, AB, CC and DD. The results revealed that the buffaloes during summer had polymorphic genotypes for MTNR1A gene as buffaloes showing reproductive cyclicity had MM-3, MM-4, RR-2, EE-3, mm-2, mm-4, rr-3, ee-3, CC and DD genotypes, whereas their non-cyclic counterparts had MM-2, MM-6, EE-4, mm-3, mm-6, AA, BB and AB genotypes. However, no such differences were recorded with respect to age at first calving. In conclusion, polymorphic genotypes of MTNR1A gene may be considered as genetic markers to identify buffaloes with better reproductive potential during summer season.

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来源期刊

Buffalo Bulletin AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

0.50

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0.00%

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审稿时长

>12 weeks

期刊介绍： Buffalo Bulletin is published quarterly in January-March, April-June, July-September and October-December. Contributions on any aspect of research or development, progress reports of projects and news on buffalo will be considered for publication in the bulletin.