PQQ依赖性可溶性葡萄糖脱氢酶催化乳糖氧化反应的电子转移介质

IF 0.5 4区化学 Q4 CHEMISTRY, MULTIDISCIPLINARY

Chemija Pub Date : 2019-09-24 DOI:10.6001/chemija.v30i3.4054

Greta Vaitkutė, I. Bratkovskaja, V. Časaitė, J. Stankevičiūtė, R. Meškys, L. Tetianec

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引用次数: 2

摘要

本工作的目的是研究人工电子转移介质在吡咯喹啉醌依赖性可溶性葡萄糖脱氢酶（PQQ-sGDH）催化的乳糖氧化反应中的应用。以1-（N，N-二甲胺）-4-（4-吗啉）苯（AMB）和N，N'-二甲基-4,4'-偶氮吡啶鎓甲基硫酸酯（MAZP）为介质。介质显示出与还原形式的PQQ-sGDH的高反应性，并用于测定pH 7.0和pH 8.0下的PQQ-s GDH催化的乳糖氧化参数。通过应用介质的电化学氧化，研究了MAZP和AMB用于再生氧化形式的PQQsGDH。当缓冲溶液pH为8.0，AMB作为电子受体在大表面积的碳纤维电极上氧化时，达到了最大的电酶乳糖生物转化产率和最大的平均PQQ-sGDH周转数。

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Electron transfer mediators for PQQ dependent soluble glucose dehydrogenase catalyzed lactose oxidation reaction

The aim of this work was to investigate the application of artificial electron transfer mediators for the pyrroloquinoline quinone-dependent soluble glucose dehydrogenase (PQQ-sGDH) catalyzed lactose oxidation reaction. 1-(N,N-dimethylamine)4-(4-morpholine)benzene (AMB) and N,N’-dimethyl-4,4’-azopyridinium methyl sulfate (MAZP) were used as mediators. The mediators showed a high reactivity with a reduced form of PQQ-sGDH and were used to determine the PQQ-sGDH catalyzed lactose oxidation parameters at pH 7.0 and pH 8.0. The use of MAZP and AMB for the regeneration of the oxidized form of PQQsGDH was investigated by applying the electrochemical oxidation of the mediator. The maximum electroenzymatic lactose bioconversion yield and the biggest average PQQ-sGDH turnover number were reached when the buffer solution pH was 8.0 and AMB was used as an electron acceptor, which was oxidized on a carbon fiber electrode of a large surface area.

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来源期刊

Chemija 化学-化学综合

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

>12 weeks

期刊介绍： Chemija publishes original research articles and reviews from all branches of modern chemistry, including physical, inorganic, analytical, organic, polymer chemistry, electrochemistry, and multidisciplinary approaches.