Anti-proliferative effects of Cuminum cyminum extraction by co-administration of layered double hydroxide (LDH) nanosheets on SW480 colorectal cancer cell line through apoptosis induction

Colorectal cancer (CC) is a common cancer in human and one of the major cause of cancer death worldwide. Recently, several therapeutic methods of CC and other malignancies have been developed, but drugs resistance is an important problem in treatment cancer treatment. Therefore, identify and provide novel chemotherapeutic agents is important for treatment of malignancies. In the present study we investigated the anti-cancer activity of Cuminum cyminum extraction in combination of layered double hydroxide (LDH) nanosheets on SW480 CC cell line. The anti-cancer activity of C. cyminum extraction and LDH nanosheets were investigated on SW480 CC cell line by MTT assay. The mRNA expression of apoptosis-related genes (BAX and BCL2) were investigated by Real-Time PCR method. Our results revealed that extraction of C. cyminum significantly decreased proliferation and viability of SW480 CC cell line as a concentration‐time dependent manner. However, anti-proliferation effects of C. cyminum extraction by co-administration of LDH nanosheets was significantly more than its monotherapy. Moreover, expression of BAX and BCL2 genes in the treated SW480 cells was significantly upregulated and downregulated, respectively. In general, present study revealed a significant anti-cancer effects of C. cyminum extraction and LDH nanosheets combination on SW480 CC cells, which may be due to apoptosis induction.