亮甲酚蓝染色提高猪卵母细胞形态质量评价的初步研究

IF 0.4 Q4 VETERINARY SCIENCES
Monika Dovenska, B. Atanasov, Michal Benc, M. Nikolovski, L. Pendovski, J. Laurinčík, T. Dovenski, N. Adamov, F. Popovska-Perčinić
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引用次数: 0

摘要

摘要亮甲蓝(BCB)染色是一种除了标准形态学分类外,还用于选择具有发育能力的猪卵母细胞的方法。本研究的目的是评估具有高和低形态特征的猪卵母细胞中BCB染色与其成熟率之间的关系。在研究的第一部分中,从60个卵巢中吸出卵丘-卵母细胞复合体(COCs)(n=434)。根据形态学分类和BCB染色,它们被分为4组:COC+/BCB+、COC-/BCB+,COC+/BCB−和COC-/BCB−。在第二个实验中,COCs(n=445)被分为3组:对照组、BCB+组和BCB-组。在COC+/BCB+和COC−/BCB-之间观察到成熟率的显著差异(66%对23%),以及COC+/BCB+和COC−/BCB+之间(66%对38%)(p<0.0001)。COC−/BCB+和COC–/BCB-之间(38%对23%)和COC+/BCB−和COC-/BCB−之间(53%对23%)也存在显著差异(p<0.01)。第二个实验的结果显示,与对照组(63%)和BCB+卵母细胞(59%)相比,只有BCB-卵母细胞的成熟率(28%)显著较低这些发现表明,与低形态的COCs相比,具有高形态特征的COCs具有更高的发育能力。与对照组相比,高质量卵母细胞中的BCB染色对其成熟能力没有显著影响,但它可能有助于选择具有低形态特征的发育能力的卵母细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Brilliant Cresyl Blue Staining Enhances the Morphological Quality Evaluation of Porcine Oocytes-a Preliminary Study
Abstract Brilliant cresyl blue (BCB) staining is a method used for selection of developmentally competent porcine oocytes, in addition to the standard morphological classification. The aim of the current study was to assess the relationship between BCB staining in porcine oocytes with high and low morphological characteristics and its maturation rate. In the first part of the study, cumulus-oocytes complexes (COCs) (n=434) were aspirated from 60 ovaries. According to the morphological classification and BCB staining, they were divided in 4 groups: COC+/BCB+, COC−/BCB+, COC+/BCB−, and COC−/BCB−. In the second experiment, COCs (n=445) were categorized into 3 groups: control, BCB+, and BCB−. Significant differences in the maturation rate were observed between COC+/BCB+ and COC−/BCB− (66% vs. 23%), and between COC+/BCB+ and COC−/BCB+ (66% vs. 38%) (p<0.0001). Significant differences were also registered between COC−/BCB+ and COC−/BCB-(38% vs. 23%) and between COC+/BCB− and COC−/BCB− (53% vs. 23%) (p<0.01). Results from the second experiment showed that only BCB− oocytes had a significantly lower maturation rate (28%) compared to the control (63%) and BCB+ oocytes (59%) (p<0.001). These findings showed that COCs with high morphological characteristics had higher developmental ability compared to COCs with low morphology. The BCB-staining in high-quality oocytes did not have a significant impact on their maturation ability compared to a control group, but it might be useful for selecting developmentally competent oocytes with low morphology characteristics.
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来源期刊
Macedonian Veterinary Review
Macedonian Veterinary Review Veterinary-Veterinary (all)
CiteScore
1.00
自引率
0.00%
发文量
20
审稿时长
12 weeks
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