组织微阵列技术在兽医学组织学中的应用

B. Ressel, A. Massone, C. Barbeito
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引用次数: 0

摘要

组织微阵列是对数十到数百个组织样本进行高通量同时分析的平台。它们特别用于分析肿瘤组织。然而,这种技术的优点可以应用于其他研究领域,如胚胎学和组织学。本研究探索了组织微阵列在动物正常组织形态学和分子分析中的应用。从产前和产后的狗身上总共选择了82个正常皮肤样本,并用于构建阵列。从该阵列块中获得大量切片,并应用常规染色和免疫组织化学技术。结果表明,该技术同时对大量正常组织样本进行形态学和分子分析是有效的。这允许对组织进行标准化评估,从而减少对单个样本进行测试时可能出现的可变性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Uso de la técnica de micromatrices de tejido en histología veterinaria
Tissue microarrays are platforms for high throughput simultaneous analysis of tens to hundreds of tissue samples. They have been used especially to analyze neoplastic tissues. However, the advantages of this technique could be applied to other research areas such as embryology and histology. This study explores the use of tissue microarrays for morphological and molecular analysis of normal tissues from animals. A total of 82 samples of normal skin from prenatal and postnatal dogs were selected and used to construct the array. A large number of sections from this array block were obtained and routine stain and immunohistochemistry techniques were applied. The results show the effectiveness of the technique for morphological and molecular analysis of a large number of normal tissue samples simultaneously. This allows the standardized assessment of tissues, thus reducing the variability that can occur when testing is performed on individual samples.
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来源期刊
CiteScore
0.30
自引率
0.00%
发文量
4
审稿时长
24 weeks
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