{"title":"从鼠尾草籽(鼠尾草)中消化的蛋白质可以防止高脂肪饮食小鼠的肥胖和相关的脂肪组织炎症","authors":"Mariana Grancieri , Thaisa Agrizzi Verediano , Cintia Tomaz Sant'Ana , Andressa de Assis , Renata Lopes Toledo , Elvira Gonzalez de Mejia , Hercia Stampini Duarte Martino","doi":"10.1016/j.phanu.2022.100298","DOIUrl":null,"url":null,"abstract":"<div><h3>Background and aim</h3><p><span>Overweight and obesity are associated with the development of several health complications, such as diabetes, cancer, and cardiovascular disease. Chia seed is a rich source of </span>proteins and peptides<span><span> with potential anti-inflammatory and antioxidant effects. This study aimed to evaluate the effects of digested proteins (DP) from chia seed to prevent adipogenesis and </span>adipose tissue inflammation in mice fed a high-fat diet.</span></p></div><div><h3>Methods and results</h3><p><span>C57Bl/6 black mice were fed a high-fat diet plus DP (400 mg/kg of body/day) for 9 weeks. DP from chia seed reduced levels of plasma total cholesterol<span><span> (−17.5%), LDL (−42.8%), triacylglycerides (−12.3%), % body fat (−26.98%), and waist circumference (−5.5%) in </span>obese mice (</span></span><em>p</em><span> < 0.05). Furthermore, treatment with DP reduced (</span><em>p</em><span><span> < 0.05) adipocytes area, foci of inflammation, levels of p-NF-κB p65, PPARγ, mRNA </span>SREBP1<span><span><span> (sterol regulatory element-binding transcription), and TNF-α. DP also increased mRNA adiponectin on adipose tissue in animals DP-treated, compared with no-DP-treated animals. However, mRNA </span>LPL and </span>HDL levels were not changed (p > 0.05). The peptides from DP had </span></span><span><em>in silico</em></span> high interaction with metalloproteinase-2.</p></div><div><h3>Conclusions</h3><p>When using this experimental model, DP from chia seed had an anti-inflammatory and anti-adipogenic effect. These results suggest the effectiveness of digested proteins from chia seed against central obesity and its associated inflammation.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"21 ","pages":"Article 100298"},"PeriodicalIF":2.4000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"8","resultStr":"{\"title\":\"Digested protein from chia seed (Salvia hispanica L) prevents obesity and associated inflammation of adipose tissue in mice fed a high-fat diet\",\"authors\":\"Mariana Grancieri , Thaisa Agrizzi Verediano , Cintia Tomaz Sant'Ana , Andressa de Assis , Renata Lopes Toledo , Elvira Gonzalez de Mejia , Hercia Stampini Duarte Martino\",\"doi\":\"10.1016/j.phanu.2022.100298\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background and aim</h3><p><span>Overweight and obesity are associated with the development of several health complications, such as diabetes, cancer, and cardiovascular disease. Chia seed is a rich source of </span>proteins and peptides<span><span> with potential anti-inflammatory and antioxidant effects. This study aimed to evaluate the effects of digested proteins (DP) from chia seed to prevent adipogenesis and </span>adipose tissue inflammation in mice fed a high-fat diet.</span></p></div><div><h3>Methods and results</h3><p><span>C57Bl/6 black mice were fed a high-fat diet plus DP (400 mg/kg of body/day) for 9 weeks. DP from chia seed reduced levels of plasma total cholesterol<span><span> (−17.5%), LDL (−42.8%), triacylglycerides (−12.3%), % body fat (−26.98%), and waist circumference (−5.5%) in </span>obese mice (</span></span><em>p</em><span> < 0.05). Furthermore, treatment with DP reduced (</span><em>p</em><span><span> < 0.05) adipocytes area, foci of inflammation, levels of p-NF-κB p65, PPARγ, mRNA </span>SREBP1<span><span><span> (sterol regulatory element-binding transcription), and TNF-α. DP also increased mRNA adiponectin on adipose tissue in animals DP-treated, compared with no-DP-treated animals. However, mRNA </span>LPL and </span>HDL levels were not changed (p > 0.05). The peptides from DP had </span></span><span><em>in silico</em></span> high interaction with metalloproteinase-2.</p></div><div><h3>Conclusions</h3><p>When using this experimental model, DP from chia seed had an anti-inflammatory and anti-adipogenic effect. These results suggest the effectiveness of digested proteins from chia seed against central obesity and its associated inflammation.</p></div>\",\"PeriodicalId\":20049,\"journal\":{\"name\":\"PharmaNutrition\",\"volume\":\"21 \",\"pages\":\"Article 100298\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PharmaNutrition\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213434422000111\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"NUTRITION & DIETETICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PharmaNutrition","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213434422000111","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
Digested protein from chia seed (Salvia hispanica L) prevents obesity and associated inflammation of adipose tissue in mice fed a high-fat diet
Background and aim
Overweight and obesity are associated with the development of several health complications, such as diabetes, cancer, and cardiovascular disease. Chia seed is a rich source of proteins and peptides with potential anti-inflammatory and antioxidant effects. This study aimed to evaluate the effects of digested proteins (DP) from chia seed to prevent adipogenesis and adipose tissue inflammation in mice fed a high-fat diet.
Methods and results
C57Bl/6 black mice were fed a high-fat diet plus DP (400 mg/kg of body/day) for 9 weeks. DP from chia seed reduced levels of plasma total cholesterol (−17.5%), LDL (−42.8%), triacylglycerides (−12.3%), % body fat (−26.98%), and waist circumference (−5.5%) in obese mice (p < 0.05). Furthermore, treatment with DP reduced (p < 0.05) adipocytes area, foci of inflammation, levels of p-NF-κB p65, PPARγ, mRNA SREBP1 (sterol regulatory element-binding transcription), and TNF-α. DP also increased mRNA adiponectin on adipose tissue in animals DP-treated, compared with no-DP-treated animals. However, mRNA LPL and HDL levels were not changed (p > 0.05). The peptides from DP had in silico high interaction with metalloproteinase-2.
Conclusions
When using this experimental model, DP from chia seed had an anti-inflammatory and anti-adipogenic effect. These results suggest the effectiveness of digested proteins from chia seed against central obesity and its associated inflammation.