印尼高本底辐射地区Takandeang村居民个体辐射敏感性评估

IF 0.6 Q4 NUCLEAR SCIENCE & TECHNOLOGY

Atom Indonesia Pub Date : 2019-04-30 DOI:10.17146/AIJ.2019.724

D. Ramadhani, S. Purnami, S. Nurhayati, M. Lubis, D. Tetriana, W. Mailana, K. D. Purwanti, E. Pudjadi, I. Kashiwakura, R. Okazaki, M. Syaifudin

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引用次数: 3

摘要

生活在高本底辐射区(HBRA)的人可能会产生辐射适应反应(RAR)现象。由于天然铀含量高，印度尼西亚西苏拉威西岛的Mamuju地区也被称为印度尼西亚的HBRA。有可能RAR是在Mamuju居民中发展起来的。为了证明这一假设，本研究使用g2微核(MN)测定法对马木居村Takandeang村居民的个体放射敏感性进行了评估。血型和TP53 Arg72Pro多态性与个体放射敏感性之间的关系也在本研究中进行了评估。采用g2mn测定法评估了Takandeang村居民和对照样本的个体放射敏感性。对于每个样本，计算三个参数。计算自发(基线)MN数、0.5 Gy体外辐照后MN数和辐射诱导MN数，预测个体放射敏感性。辐射诱导MN的定义是用辐照后MN数减去自发MN数。以对照组(CG)辐射诱发微核数的均值和标准差作为截止值，确定各样本的个体辐射敏感性。高于CG + 1SD CS平均值的辐射诱导MN评分为1，表明放射敏感表型较轻，高于CG + 2SD CS平均值的结果评分为2，表明放射敏感表型较严重。当个体值低于平均CG + 1SD CG时，被试得分为0。结果显示，Takandeang村居民中有4人具有轻度放射敏感表型，而另一人属于正常放射敏感表型。在对照样本中也发现了类似的结果。我们的研究没有发现放射敏感性与血型以及TP53 Arg72Pro多态性之间的相关性。总体而言，我们的研究揭示了在Takandeang村居民中存在RAR现象的可能性。进一步研究应采用不同的辐射剂量值点和更大的样本数来验证本研究结果。

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Assessment of Individual Radiosensitivity in Inhabitants of Takandeang Village － A High Background Radiation Area in Indonesia

People living in high background radiation area (HBRA) possibly developed the radioadaptive response (RAR) phenomena. Mamuju area in West Sulawesi Indonesia also known as the HBRA in Indonesia due to high natural uranium contents. It is possible that RAR was developed in Mamuju inhabitants. To prove this hypothesis, here in this study evaluation of the individual radiosensitivity in Takandeang Village, Mamuju inhabitants using G 2 micronucleus (MN) assay was conducted. Association between blood groups and TP53 Arg72Pro polymorphism with individual radiosensitivity also evaluated in this study. Using G 2 MN assay we assessed the individual radiosensitivity of Takandeang Village inhabitants and control samples. For each sample, three parameters were calculated. The spontaneous (baseline) MN number, MN number after 0.5 Gy in vitro irradiation and radiation induced MN were calculated to predict the individual radiosensitivity. The radiation induced MN was deﬁned by subtracting the spontaneous MN number from the MN number after irradiation. The mean and SD of the number of micronucleus induced by radiation found in control group (CG) was set as the cut-off value to determine the individual radiosensitivity in all samples. The radiation induced MN higher than the mean CG + 1SD CS was scored as 1, indicating a milder radiosensitive phenotype, whereas a result higher than the mean CG + 2SD CG was scored as 2, and indicated a more severe radiosensitive phenotype. When the individual value was lower than the mean CG + 1SD CG, a score of 0 was attributed to the tested subject. Results showed that four individuals in Takandeang Village inhabitants were having a milder radiosensitive phenotype, whilst the other was categorized as normal radiosensitive. The similar finding also found in control samples. Our study failed to find a correlation between radiosensitivity and blood group also the TP53 Arg72Pro polymorphism. Overall our study revealed the possibility of RAR phenomena in Takandeang Village inhabitants. Further investigation using a different point of radiation dose value and larger sample number should be performed to validate this study results.

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来源期刊

Atom Indonesia NUCLEAR SCIENCE & TECHNOLOGY-

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

16 weeks

期刊介绍： The focus of Atom Indonesia is research and development in nuclear science and technology. The scope of this journal covers experimental and analytical research in nuclear science and technology. The topics include nuclear physics, reactor physics, radioactive waste, fuel element, radioisotopes, radiopharmacy, radiation, and neutron scattering, as well as their utilization in agriculture, industry, health, environment, energy, material science and technology, and related fields.