京尼平苷通过调控PPARγ/VEGF-A通路抑制非小细胞肺癌细胞迁移和血管生成

IF 4.6 3区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Quality Assurance and Safety of Crops & Foods Pub Date : 2022-01-21 DOI:10.15586/qas.v14i1.1016

Ming Jiang, S. Zheng

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引用次数: 2

摘要

栀子苷是一种从栀子中提取的环烯醚萜苷类化合物，对多种肿瘤具有抗肿瘤作用。本研究探讨了京尼平苷在非小细胞肺癌(NSCLC)细胞迁移和血管生成中的作用。用不同浓度的京尼平苷培养癌细胞，通过细胞计数试剂盒-8 (CCK-8)和5-乙基-2′-脱氧尿苷(EdU)染色检测癌细胞的增殖能力。伤口愈合和transwell分别用于评估细胞迁移和侵袭。血管形成实验观察血管生成情况。京尼平苷降低非小细胞肺癌细胞增殖，抑制非小细胞肺癌细胞迁移和侵袭，呈剂量依赖性。京尼平苷对非小细胞肺癌血管生成也有抑制作用。京尼平苷增加了NSCLC细胞中过氧化物酶体增殖物激活受体γ (PPARγ)的蛋白表达，降低了血管内皮生长因子-a (VEGF-A)蛋白表达。与PPARγ拮抗剂GW9662孵育，减弱了京尼平苷诱导的PPARγ上调和VEGF-A下调。VEGF-A过表达削弱了京尼平苷抑制的非小细胞肺癌细胞增殖、迁移和血管生成。京尼平苷通过调控PPARγ/VEGF-A通路对NSCLC发挥抗肿瘤和抗血管生成作用。

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Geniposide inhibits non-small cell lung cancer cell migration and angiogenesis by regulating PPARγ/VEGF-A pathway

Geniposide, an iridoid glycoside derived from traditional Chinese herb, Gardenia jasminoides Ellis, exerts antitumor effect against distinct cancers. The role of geniposide in the migration and angiogenesis of non-small cell lung cancer (NSCLC) cell was investigated in this study. Cancer cells were incubated with various concentrations of geniposide, and proliferative ability was detected by Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) staining. Wound healing and transwell were used to assess cell migration and invasion, respectively. Tube formation assay was performed to investigate angiogenesis. Geniposide reduced NSCLC cell proliferation, and suppressed NSCLC cell migration and invasion in a dosage-dependent manner. Angiogenesis of NSCLC was also inhibited by geniposide. Geniposide increased the protein expression of peroxisome proliferator-activated receptor gamma (PPARγ) and decreased vascular endothelial growth factor-A (VEGF-A) protein expression in NSCLC cells. Incubation with a PPARγ antagonist, GW9662, attenuated geniposide-induced up-regulation of PPARγ and down-regulation of VEGF-A. Over-expression of VEGF-A weakened geniposide-suppressed cell proliferation, migration, and angiogenesis of NSCLC. Geniposide exerted antitumor and anti-angiogenic actions on NSCLC through regulation of PPARγ/VEGF-A pathway.

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来源期刊

Quality Assurance and Safety of Crops & Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

4.60

自引率

7.50%

发文量

审稿时长

1 months

期刊介绍： ''Quality Assurance and Safety of Crops & Foods'' is an international peer-reviewed journal publishing research and review papers associated with the quality and safety of food and food sources including cereals, grains, oilseeds, fruits, root crops and animal sources. It targets both primary materials and their conversion to human foods. There is a strong focus on the development and application of new analytical tools and their potential for quality assessment, assurance, control and safety. The scope includes issues of risk assessment, traceability, authenticity, food security and socio-economic impacts. Manuscripts presenting novel data and information that are likely to significantly contribute to scientific knowledge in areas of food quality and safety will be considered. ''Quality Assurance and Safety of Crops & Foods'' provides a forum for all those working in the specialist field of food quality and safety to report on the progress and outcomes of their research.