丹参乙醇根提取物对白化大鼠的植物化学分析及安全性评价

O. M. Mwanasiti, A. Florence, C. K. Mutinda, N. Esther
{"title":"丹参乙醇根提取物对白化大鼠的植物化学分析及安全性评价","authors":"O. M. Mwanasiti, A. Florence, C. K. Mutinda, N. Esther","doi":"10.5897/jmpr2021.7204","DOIUrl":null,"url":null,"abstract":"Erythrina sacleuxii , one of five Erythrina species found in Kenya, is a stiffly multi-branched deciduous tree reported to have antiplasmodial, antifungal, anti-bacterial and anti-cancer activities. This study aimed at evaluating acute and sub-acute oral toxicity of E. sacleuxii roots ethanolic extract. Phytochemical analysis was conducted on Fourier transform infrared spectroscopy and Gas Chromatography-Mass Spectrometer. For acute and sub-acute toxicity studies 16 male rats n=4/group were used. The control group received 2% DMSO while treatment groups received 400, 1200 and 2400 mg/kg single dose (acute toxicity assay) and repeated for 14 days in subacute toxicity assay. Histopathological, serum biochemistry and hematological tests were analyzed. The results of FT-IR analysis recorded 12 dominant peaks, confirming availability of N-H, C-N, -C=C-, C-H, S=O, C-O and C-C functional groups. GC-MS analysis revealed presence of 17 bioactive phytochemicals mainly terpenoids and phenols. No toxic effect, organ toxicity or death was recorded in acute toxicity assay. In the subacute toxicity investigation, rats' behavior, gross pathology, and hematological parameters showed no significant changes. Biochemical analysis indicated an increase in alkaline phosphatase from 147 U/L at 400 mg/kg to 164 U/L at 2400 mg/kg however this was not statistically significant and no mortality was recorded. In comparison to the negative control, treatment groups’ gross and histopathology of livers and kidneys had no significant difference. These results demonstrate that 80% ethanolic extract of E. sacleuxii roots has no severe effects in both acute and subacute study. However, sub-chronic and chronic toxicity studies are necessary for further validation. ,","PeriodicalId":16387,"journal":{"name":"Journal of Medicinal Plants Research","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phytochemical analysis and safety evaluation of ethanol roots extract of Erythrina sacleuxii hua in Wistar albino rats\",\"authors\":\"O. M. Mwanasiti, A. Florence, C. K. Mutinda, N. Esther\",\"doi\":\"10.5897/jmpr2021.7204\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Erythrina sacleuxii , one of five Erythrina species found in Kenya, is a stiffly multi-branched deciduous tree reported to have antiplasmodial, antifungal, anti-bacterial and anti-cancer activities. This study aimed at evaluating acute and sub-acute oral toxicity of E. sacleuxii roots ethanolic extract. Phytochemical analysis was conducted on Fourier transform infrared spectroscopy and Gas Chromatography-Mass Spectrometer. For acute and sub-acute toxicity studies 16 male rats n=4/group were used. The control group received 2% DMSO while treatment groups received 400, 1200 and 2400 mg/kg single dose (acute toxicity assay) and repeated for 14 days in subacute toxicity assay. Histopathological, serum biochemistry and hematological tests were analyzed. The results of FT-IR analysis recorded 12 dominant peaks, confirming availability of N-H, C-N, -C=C-, C-H, S=O, C-O and C-C functional groups. GC-MS analysis revealed presence of 17 bioactive phytochemicals mainly terpenoids and phenols. No toxic effect, organ toxicity or death was recorded in acute toxicity assay. In the subacute toxicity investigation, rats' behavior, gross pathology, and hematological parameters showed no significant changes. Biochemical analysis indicated an increase in alkaline phosphatase from 147 U/L at 400 mg/kg to 164 U/L at 2400 mg/kg however this was not statistically significant and no mortality was recorded. In comparison to the negative control, treatment groups’ gross and histopathology of livers and kidneys had no significant difference. These results demonstrate that 80% ethanolic extract of E. sacleuxii roots has no severe effects in both acute and subacute study. However, sub-chronic and chronic toxicity studies are necessary for further validation. ,\",\"PeriodicalId\":16387,\"journal\":{\"name\":\"Journal of Medicinal Plants Research\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-04-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Medicinal Plants Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5897/jmpr2021.7204\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Medicinal Plants Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5897/jmpr2021.7204","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

sacleuxii Erythrina sacleuxii是肯尼亚发现的五种Erythrina之一,是一种刚硬多枝落叶乔木,据报道具有抗疟原虫、抗真菌、抗菌和抗癌活性。本研究旨在评价枫香根乙醇提取物的急性和亚急性口服毒性。采用傅里叶变换红外光谱和气相色谱-质谱联用仪进行植物化学分析。急性和亚急性毒性研究采用16只雄性大鼠,每组4只。对照组给予2% DMSO,治疗组给予400、1200、2400 mg/kg单剂量(急性毒性试验),亚急性毒性试验重复14 d。进行组织病理学、血清生化及血液学检查。FT-IR分析结果记录了12个优势峰,证实了N-H、C- n、C=C-、C- h、S=O、C-O和C-C官能团的可用性。GC-MS分析显示其含有17种植物活性化学物质,主要是萜类和酚类。急性毒性试验未见毒副作用、器官毒性及死亡。在亚急性毒性研究中,大鼠的行为、大体病理和血液学参数未见明显变化。生化分析表明,碱性磷酸酶从400 mg/kg时的147 U/L增加到2400 mg/kg时的164 U/L,但这没有统计学意义,也没有死亡记录。与阴性对照组相比,各治疗组肝脏和肾脏的大体和组织病理学无显著差异。以上结果表明,80%乙醇提取物在急性和亚急性研究中均无严重影响。然而,亚慢性和慢性毒性研究是进一步验证的必要条件。,
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phytochemical analysis and safety evaluation of ethanol roots extract of Erythrina sacleuxii hua in Wistar albino rats
Erythrina sacleuxii , one of five Erythrina species found in Kenya, is a stiffly multi-branched deciduous tree reported to have antiplasmodial, antifungal, anti-bacterial and anti-cancer activities. This study aimed at evaluating acute and sub-acute oral toxicity of E. sacleuxii roots ethanolic extract. Phytochemical analysis was conducted on Fourier transform infrared spectroscopy and Gas Chromatography-Mass Spectrometer. For acute and sub-acute toxicity studies 16 male rats n=4/group were used. The control group received 2% DMSO while treatment groups received 400, 1200 and 2400 mg/kg single dose (acute toxicity assay) and repeated for 14 days in subacute toxicity assay. Histopathological, serum biochemistry and hematological tests were analyzed. The results of FT-IR analysis recorded 12 dominant peaks, confirming availability of N-H, C-N, -C=C-, C-H, S=O, C-O and C-C functional groups. GC-MS analysis revealed presence of 17 bioactive phytochemicals mainly terpenoids and phenols. No toxic effect, organ toxicity or death was recorded in acute toxicity assay. In the subacute toxicity investigation, rats' behavior, gross pathology, and hematological parameters showed no significant changes. Biochemical analysis indicated an increase in alkaline phosphatase from 147 U/L at 400 mg/kg to 164 U/L at 2400 mg/kg however this was not statistically significant and no mortality was recorded. In comparison to the negative control, treatment groups’ gross and histopathology of livers and kidneys had no significant difference. These results demonstrate that 80% ethanolic extract of E. sacleuxii roots has no severe effects in both acute and subacute study. However, sub-chronic and chronic toxicity studies are necessary for further validation. ,
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
25
审稿时长
4.3 months
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信