来自平菇的β-(1,3)- d -葡聚糖与高脂高果糖饮食(HFFD)大鼠血浆IL-6、IL-1β、HOMA-IR和更高的胰腺β细胞计数相关

IF 0.2 Q4 HEALTH CARE SCIENCES & SERVICES
Alma Maghfirotun Innayah, Elvira Nur Sa’idah Hariani, H. Khotimah, I. Kusumastuty, E. Yunita, D. Handayani
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引用次数: 0

摘要

导读:高脂肪和高果糖食品消费的增加导致全球肥胖患病率的上升。肥胖患者的轻度慢性炎症是胰岛素抵抗和2型糖尿病的重要危险因素。因此,本研究旨在研究从平菇(Pleurotus ostreatus)中提取的β-(1,3)- d -葡聚糖对高脂高果糖饮食大鼠的影响。设计与方法:以35只8周龄雄性Sprague-Dawley大鼠为实验对象。将大鼠分为正常组(N)、高脂高果糖组(HFFD)、D1组(HFFD+125 mg/kg BW β-葡聚糖)、D2组(HFFD+250 mg/kg BW β-葡聚糖)、D3组(HFFD+375 mg/kg BW β-葡聚糖),干预14周。采用ELISA法测定IL-6、IL-1β水平,采用空腹胰岛素(ng/mL) ×空腹血糖(mg/dL)/405计算HOMA-IR(胰岛素抵抗稳态模型评估)。苏木精和伊红(H&E)染色测定胰腺β细胞计数。结果:两组间IL-6、IL-1β水平无明显差异。然而,两组间HOMA-IR和胰腺β细胞计数有显著差异。β-葡聚糖剂量与IL-6、IL-1β、HOMA-IR水平呈负相关。此外,β-葡聚糖的剂量与胰腺β细胞的数量呈正相关。结论:在不影响体重增加的情况下,给予平菇提取物β-(1,3)- d -葡聚糖可预防高血糖和胰岛素抵抗,并减轻炎症反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
β-(1,3)-D-glucan from Pleurotus ostreatus correlates with lower plasma IL-6, IL-1β, HOMA-IR, and higher pancreatic beta cell count in High-Fat and High-Fructose Diet (HFFD) rats
Introduction: The increasing consumption of high-fat and high-fructose foods contributes to the increasing prevalence of global obesity. Low-grade chronic inflammation in obesity is a significant risk factor for insulin resistance and type 2 diabetes. Therefore, this study aimed to determine the effect of β-(1,3)-D-glucan from oyster mushroom (Pleurotus ostreatus) extract on rats fed with a high-fat and high-fructose diet. Design and Methods: This experimental study was conducted on 35 male Sprague-Dawley rats aged eight weeks. The rats were divided into groups given a normal (N) diet, a high-fat and high-fructose diet (HFFD), D1 (HFFD+125 mg/kg BW β-glucan), D2 (HFFD+250 mg/kg BW β glucan), and D3 (HFFD+375 mg/kg BW β-glucan) with an intervention of 14 weeks. IL-6 and IL-1β levels were measured by the ELISA method, while HOMA-IR (Homeostatic Model Assessment for Insulin Resistance) was calculated by the fasting insulin (ng/mL) x fasting blood glucose (mg/dL)/405 formula. Pancreatic beta-cell counts were measured by hematoxylin and eosin (H&E) staining. Results: The results showed no differences in IL-6 and IL-1β between the treatment groups. However, there were significant differences in HOMA-IR and pancreatic beta-cell counts between groups. There were negative correlations between the dose of β-glucan and IL-6, IL-1β, and HOMA-IR levels. Also, there was a positive correlation between the dose of β-glucan and the number of pancreatic beta cells. Conclusions: Administration of β-(1,3)-D-glucan from oyster mushroom (Pleurotus ostreatus) extract prevented hyperglycemia and insulin resistance, also reduced inflammation in rats fed with HFFD regardless of weight gain.
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CiteScore
0.10
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发文量
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审稿时长
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