基于基因分型测序(GBS) SNP标记的大豆高密度一致遗传图谱的构建

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2022-08-01 Epub Date: 2022-06-03 DOI:10.1139/gen-2021-0054
Manel Fallah, Martine Jean, Vincent-Thomas Boucher St-Amour, Louise O'Donoughue, François Belzile
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引用次数: 1

摘要

遗传连锁图谱是基于重组频率对基因组标记进行定位的方法。大多数情况下,这些图谱是基于在一个规模有限的双亲群体(n<300)中观察到的分离,其中重组事件相对较少,并且由于双亲携带相同的等位基因,一些基因组区域是单态的。总之,这两个限制影响了这些图谱的分辨率和基因组覆盖范围。共识遗传图谱通过合并来自亲本组合多样性更高的多个分离群体的信息,克服了个体遗传图谱的局限性,从而增加了重组事件的数量,减少了单态区域的数量。本研究的目的是为通过测序基因分型(GBS)方法获得的SNP标记构建高密度共识遗传图谱。6个F4:5作图群体(n=278 ~ 365)共1857个个体生成了个体遗传图谱。然后将这6个连锁图谱合并产生一个共识图谱,其中包括16311个已绘制的snp,这些snp共同覆盖了99.5%的大豆基因组,只有两个大于10 cM的缺口。与以前的大豆共识图相比,它提供了更广泛和统一的覆盖范围。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The construction of a high-density consensus genetic map for soybean based on SNP markers derived from genotyping-by-sequencing.

Genetic linkage maps are used to localize markers on the genome based on the recombination frequency. Most often, these maps are based on the segregation observed within a single biparental population of limited size (n < 300) where relatively few recombination events are sampled and in which some genomic regions are monomorphic because both parents carry the same alleles. Together, these two limitations affect both the resolution and extent of genome coverage of such maps. Consensus genetic maps overcome the limitations of individual genetic maps by merging the information from multiple segregating populations derived from a greater diversity of parental combinations, thus increasing the number of recombination events and reducing the number of monomorphic regions. The aim of this study was to construct a high-density consensus genetic map for single nucleotide polymorphism (SNP) markers obtained through a genotyping-by-sequencing (GBS) approach. Individual genetic maps were generated from six F4:5 mapping populations (n = 278-365), totaling 1857 individuals. The six linkage maps were then merged to produce a consensus map comprising a total of 16 311 mapped SNPs that jointly cover 99.5% of the soybean genome with only two gaps larger than 10 cM. Compared to previous soybean consensus maps, it offers a more extensive and uniform coverage.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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