Andrey Carlos do Sacramento de Oliveira, Bárbara Cristina Amorim Ferreira, G. Ferreira, Andreia Silva da Silva, Milena Martins Andrade Fernandes, Josyane Brasil da Silva, Karla Fernanda Viana Mendonça Jardim, E. M. Dias, F. Leite, T. Roos, C. M. Moraes
{"title":"多重聚合酶链式反应同时检测马拉岛销售的碎牛肉中的牛牛、布巴鲁和沙门氏菌的评价","authors":"Andrey Carlos do Sacramento de Oliveira, Bárbara Cristina Amorim Ferreira, G. Ferreira, Andreia Silva da Silva, Milena Martins Andrade Fernandes, Josyane Brasil da Silva, Karla Fernanda Viana Mendonça Jardim, E. M. Dias, F. Leite, T. Roos, C. M. Moraes","doi":"10.56825/bufbu.2022.4143083","DOIUrl":null,"url":null,"abstract":"The present work intends to use a mPCR for simultaneous detection of Bos taurus, Bubalus bubalis and Salmonella spp. in samples commercially available on Marajó Island, Pará State, Brazil. For this purpose, a mPCR reaction with different concentrations of DNA and primers with different annealing temperatures was optimized. Next, 28 samples of ground meat marketed as being of bovine origin were collected and a mPCR for the determination of species and for the detection of Salmonella spp. were realized. The results demonstrated the efficiency of the proposed technique for identification of Bos taurus, Bubalus bubalis and Salmonella spp. We conclude that the proposed mPCR technique is efficient and that buffalo meat is marketed as bovine on the Island of Marajó and that such frauds violating the current legislation that normalizes the commercialization of ground beef.","PeriodicalId":9393,"journal":{"name":"Buffalo Bulletin","volume":null,"pages":null},"PeriodicalIF":0.2000,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of a multiplex PCR for simultaneous detection of Bos taurus, Bubalus bubalis and Salmonella spp. in ground beef marketed in Marajó island\",\"authors\":\"Andrey Carlos do Sacramento de Oliveira, Bárbara Cristina Amorim Ferreira, G. Ferreira, Andreia Silva da Silva, Milena Martins Andrade Fernandes, Josyane Brasil da Silva, Karla Fernanda Viana Mendonça Jardim, E. M. Dias, F. Leite, T. Roos, C. M. Moraes\",\"doi\":\"10.56825/bufbu.2022.4143083\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The present work intends to use a mPCR for simultaneous detection of Bos taurus, Bubalus bubalis and Salmonella spp. in samples commercially available on Marajó Island, Pará State, Brazil. For this purpose, a mPCR reaction with different concentrations of DNA and primers with different annealing temperatures was optimized. Next, 28 samples of ground meat marketed as being of bovine origin were collected and a mPCR for the determination of species and for the detection of Salmonella spp. were realized. The results demonstrated the efficiency of the proposed technique for identification of Bos taurus, Bubalus bubalis and Salmonella spp. We conclude that the proposed mPCR technique is efficient and that buffalo meat is marketed as bovine on the Island of Marajó and that such frauds violating the current legislation that normalizes the commercialization of ground beef.\",\"PeriodicalId\":9393,\"journal\":{\"name\":\"Buffalo Bulletin\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2022-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Buffalo Bulletin\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.56825/bufbu.2022.4143083\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Buffalo Bulletin","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.56825/bufbu.2022.4143083","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Evaluation of a multiplex PCR for simultaneous detection of Bos taurus, Bubalus bubalis and Salmonella spp. in ground beef marketed in Marajó island
The present work intends to use a mPCR for simultaneous detection of Bos taurus, Bubalus bubalis and Salmonella spp. in samples commercially available on Marajó Island, Pará State, Brazil. For this purpose, a mPCR reaction with different concentrations of DNA and primers with different annealing temperatures was optimized. Next, 28 samples of ground meat marketed as being of bovine origin were collected and a mPCR for the determination of species and for the detection of Salmonella spp. were realized. The results demonstrated the efficiency of the proposed technique for identification of Bos taurus, Bubalus bubalis and Salmonella spp. We conclude that the proposed mPCR technique is efficient and that buffalo meat is marketed as bovine on the Island of Marajó and that such frauds violating the current legislation that normalizes the commercialization of ground beef.
期刊介绍:
Buffalo Bulletin is published quarterly in January-March, April-June, July-September and October-December. Contributions on any aspect of research or development, progress reports of projects and news on buffalo will be considered for publication in the bulletin.