多重聚合酶链式反应同时检测马拉岛销售的碎牛肉中的牛牛、布巴鲁和沙门氏菌的评价

IF 0.2 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Buffalo Bulletin Pub Date : 2022-12-30 DOI:10.56825/bufbu.2022.4143083

Andrey Carlos do Sacramento de Oliveira, Bárbara Cristina Amorim Ferreira, G. Ferreira, Andreia Silva da Silva, Milena Martins Andrade Fernandes, Josyane Brasil da Silva, Karla Fernanda Viana Mendonça Jardim, E. M. Dias, F. Leite, T. Roos, C. M. Moraes

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引用次数: 0

摘要

本工作旨在使用mPCR同时检测巴西帕拉州马拉约岛市售样本中的牛牛、布巴鲁和沙门氏菌。为此，优化了具有不同浓度的DNA和具有不同退火温度的引物的mPCR反应。接下来，收集了28份以牛源销售的绞肉样品，并实现了用于确定物种和检测沙门氏菌属的mPCR。研究结果证明了所提出的鉴定牛牛、水牛和沙门氏菌的技术的有效性。我们得出的结论是，所提出的mPCR技术是有效的，水牛肉在马拉约岛上以牛的身份出售，这种欺诈行为违反了使碎牛肉商业化正常化的现行立法。

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Evaluation of a multiplex PCR for simultaneous detection of Bos taurus, Bubalus bubalis and Salmonella spp. in ground beef marketed in Marajó island

The present work intends to use a mPCR for simultaneous detection of Bos taurus, Bubalus bubalis and Salmonella spp. in samples commercially available on Marajó Island, Pará State, Brazil. For this purpose, a mPCR reaction with different concentrations of DNA and primers with different annealing temperatures was optimized. Next, 28 samples of ground meat marketed as being of bovine origin were collected and a mPCR for the determination of species and for the detection of Salmonella spp. were realized. The results demonstrated the efficiency of the proposed technique for identification of Bos taurus, Bubalus bubalis and Salmonella spp. We conclude that the proposed mPCR technique is efficient and that buffalo meat is marketed as bovine on the Island of Marajó and that such frauds violating the current legislation that normalizes the commercialization of ground beef.

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来源期刊

Buffalo Bulletin AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

0.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Buffalo Bulletin is published quarterly in January-March, April-June, July-September and October-December. Contributions on any aspect of research or development, progress reports of projects and news on buffalo will be considered for publication in the bulletin.