豆腐生产过程中大豆蛋白制品中β-淀粉酶和脂氧合酶的分布

IF 0.8 4区工程技术 Q4 ENGINEERING, CHEMICAL

Hemijska Industrija Pub Date : 2017-05-13 DOI:10.2298/HEMIND150525021S

P. Stanojevic, B. Barac, B. Pešić, V. B. Vucelic-Radovic

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引用次数: 7

摘要

大豆被认为是人类食物和动物饲料的重要来源。豆渣和豆腐乳清是豆浆和豆腐生产的主要副产品。研究了六种大豆基因型的b-淀粉酶和脂氧合酶（Lox）在豆渣和豆腐蛋白提取物以及豆浆和豆腐乳清中的分布。SDS电泳结果表明，生产工艺对大豆副产品的高分子量蛋白质组分有显著影响。豆浆中的Lox含量较低，而在豆渣蛋白提取物和豆腐乳清中，这种酶存在于微量中。在豆腐蛋白提取物中，Lox的含量较高，这可能是豆腐凝胶形成过程中聚集过程的结果。所有大豆蛋白制品中b-淀粉酶的含量都很高，与大豆基因型的含量相似。豆渣中b-淀粉酶的含量取决于不同的大豆基因型。

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Distribution of β-amylase and lipoxygenase in soy protein products obtained during tofu production

Soybean is considered an important source of human food and animal feed. Okara and tofu whey are the main by-products of soymilk and tofu production. The distribution of enzymes b -amylase and lipoxygenase (Lox) from six soybean genotypes in protein extracts of okara and tofu as well as in soymilk and tofu whey was assessed. SDS-electrophoretic results showed that production process significantly affected high molecular mass protein fractions of soybean by-products. Low amounts of Lox in soymilk were registered, while in okara protein extracts and tofu whey this enzyme was present in trace. In tofu protein extracts, Lox was registered in higher quantity that might be the result of the aggregation process during formation of the tofu gel. Content of b -amylase in all soy protein products was high and similar to the content in soybean genotype. Content of b -amylase in okara depended on respective soybean genotype.

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来源期刊

Hemijska Industrija 工程技术-工程：化工

CiteScore

1.60

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal Hemijska industrija (abbreviation Hem. Ind.) is publishing papers in the field of Chemical Engineering (Transport phenomena; Process Modeling, Simulation and Optimization; Thermodynamics; Separation Processes; Reactor Engineering; Electrochemical Engineering; Petrochemical Engineering), Biochemical Engineering (Bioreactors; Protein Engineering; Kinetics of Bioprocesses), Engineering of Materials (Polymers; Metal materials; Non-metal materials; Biomaterials), Environmental Engineeringand Applied Chemistry. The journal is published bimonthly by the Association of Chemical Engineers of Serbia (a member of EFCE - European Federation of Chemical Engineering). In addition to professional articles of importance to industry, scientific research papers are published, not only from our country but from all over the world. It also contains topics such as business news, science and technology news, information on new apparatus and equipment, and articles on environmental protection.