Еfficiency模拟感染性关节炎条件下急性期蛋白和降钙素原的测定

S. Magomedov
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The following model of drug administration was used for the experimental groups: a single daily injection of 0.02 ml of flosteron into the knee joint for seven days (group II); daily single administration for seven days of 0.02 ml of S.aureus 108 No. 209 (III group); daily one-time alternating (every other day) administration for seven days of 0.02 ml of flosteron and 0.02 ml of S.aureus 108 No. 209 into the knee joint (group IV). The effectiveness of the drugs was observed 3 and 14 days after administration. Results. It was established that the concentration of haptoglobin was significantly increased in the blood serum of rats both after 3 and 14 days in all studied groups of animals compared to the control. The greatest increase relative to the control values was noted 3 days after the seven-time injection of S.aureus 108 #209 into the knee joint. However, after 14 days it was already not so significant and significantly lower (by 85.33%) compared to the measurement after three days. Only in rats after a 14-day alternating (every other day) injection of 0.02 ml of flosteron and 0.02 ml of S.aureus 108 No. 209 into the knee joint was observed a probable increase in the level of haptoglobin by 775.08% (Р<0.05) compared to the control and 77.78% reduced compared to the measurement after three days. The concentration of ceruloplasmin in blood serum increased in all experimental rats during the entire observation period and differed little between 3 and 14 days. The content of C-reactive protein in blood serum increased in all studied groups of rats without exception, which proves its high specificity for detecting inflammatory processes of various severity. The concentration of procalcitonin was most likely to increase by 235.0% 3 days after alternating (every other day) administration of 0.02 ml of flosterone and 0.02 ml of S.aureus 108 No. 209. It was slightly lower by 120.0% under the same conditions experiment after 14 days. This indicator probably increased by 65% 14 days after the 7-time introduction of S.aureus 108 #209. In the rest of the experimental animals, the PCT concentration did not change. Conclusions. The determination of haptoglobin reflects, first of all, the primary activation of the inflammatory process, which was enhanced by the hormonal drug flosteron. However, its determination can be effective over a longer period of time, as several factors lead to a bacterial infection, reinforcing each other. At the same time, the synthesis of ceruloplasmin increases precisely during the first three days of the infectious process, which turns it into an effective marker for detecting early infectious complications. 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引用次数: 1

摘要

急性期蛋白铜蓝蛋白、触珠蛋白、C-反应蛋白(CRP)和降钙素原是表征炎症过程的标志物。C反应蛋白是急性期(AF)的核心成分之一,也是公认的炎症过程指标。目标在模拟感染性关节炎的条件下测定急性期蛋白(CRP、触珠蛋白、铜蓝蛋白)以及降钙素原的水平和效率。材料和方法。对52只白色雄性Wistar大鼠进行了实验研究。通过每天向大鼠膝关节注射0.02ml金黄色葡萄球菌108号209,建立感染性关节炎模型7天。这些动物被分为小组和动物饲养场对照组。实验组采用以下给药模式:每天向膝关节内单次注射0.02毫升氟甾酮,持续7天(第II组);每日单次给药0.02ml金黄色葡萄球菌108号209,持续7天(III组);每日一次(每隔一天)向膝关节内交替给药0.02毫升氟甾酮和0.02毫升金黄色葡萄球菌108号209,持续7天(第IV组)。在给药后3天和14天观察药物的有效性。后果已经确定,与对照组相比,在所有研究的动物组中,大鼠血清中的触珠蛋白浓度在3天和14天后都显著增加。在将金黄色葡萄球菌108#209七次注射到膝关节后3天,观察到相对于对照值的最大增加。然而,在14天后,它已经不那么显著了,并且与三天后的测量相比显著降低(85.33%)。仅在大鼠膝关节内交替(每隔一天)注射0.02毫升氟甾酮和0.02毫升金黄色葡萄球菌108号209 14天后,观察到触珠蛋白水平可能比对照组增加775.08%(Р<0.05),与三天后的测量值相比降低77.78%。在整个观察期内,所有实验大鼠血清中铜蓝蛋白的浓度都有所增加,在3至14天之间差异不大。所有研究组大鼠血清中C反应蛋白的含量无一例外地增加,这证明了它对检测各种严重程度的炎症过程具有很高的特异性。在交替(每隔一天)给药0.02毫升氟甾酮和0.02毫升金黄色葡萄球菌108号209后3天,降钙素原的浓度最有可能增加235.0%。在相同的实验条件下,14天后它稍微降低了120.0%。在7次引入金黄色葡萄球菌108#209后14天,该指标可能增加了65%。在其余实验动物中,PCT浓度没有变化。结论。触珠蛋白的测定首先反映了炎症过程的初级激活,而激素药物氟甾酮增强了炎症过程。然而,它的测定可能在更长的时间内有效,因为有几个因素会导致细菌感染,相互加强。同时,铜蓝蛋白的合成在感染过程的前三天精确增加,这使其成为检测早期感染并发症的有效标志物。血清中C反应蛋白水平的动态变化与感染过程的活性相关性最高,这证明了它在检测各种严重程度的炎症过程、选择适当的治疗和预测疾病进程方面是高效的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ЕFFICIENCY OF DETERMINATION OF ACUTE PHASE PROTEINS AND PROCALCITONIN UNDER THE CONDITIONS OF SIMULATING INFECTIOUS ARTHRITIS
Acute phase proteins ceruloplasmin, haptoglobin, C-reactive protein (CRP) and procalcitonin are markers that characterize the inflammatory process. C-reactive protein is one of the central components of the acute phase (AF) and is a generally accepted indicator of inflammatory processes. Aim. Determination of the level and efficiency of determination of acute-phase proteins (CRP, haptoglobin, ceruloplasmin), as well as procalcitonin under the conditions of modeling infectious arthritis. Materials and methods. Experimental studies were conducted on 52 white male Wistar rats. A model of infectious arthritis was created for seven days by daily injection of 0.02 ml of S.aureus 108 No. 209 into the knee joint of a rat. The animals were divided into groups - and vivarium control. The following model of drug administration was used for the experimental groups: a single daily injection of 0.02 ml of flosteron into the knee joint for seven days (group II); daily single administration for seven days of 0.02 ml of S.aureus 108 No. 209 (III group); daily one-time alternating (every other day) administration for seven days of 0.02 ml of flosteron and 0.02 ml of S.aureus 108 No. 209 into the knee joint (group IV). The effectiveness of the drugs was observed 3 and 14 days after administration. Results. It was established that the concentration of haptoglobin was significantly increased in the blood serum of rats both after 3 and 14 days in all studied groups of animals compared to the control. The greatest increase relative to the control values was noted 3 days after the seven-time injection of S.aureus 108 #209 into the knee joint. However, after 14 days it was already not so significant and significantly lower (by 85.33%) compared to the measurement after three days. Only in rats after a 14-day alternating (every other day) injection of 0.02 ml of flosteron and 0.02 ml of S.aureus 108 No. 209 into the knee joint was observed a probable increase in the level of haptoglobin by 775.08% (Р<0.05) compared to the control and 77.78% reduced compared to the measurement after three days. The concentration of ceruloplasmin in blood serum increased in all experimental rats during the entire observation period and differed little between 3 and 14 days. The content of C-reactive protein in blood serum increased in all studied groups of rats without exception, which proves its high specificity for detecting inflammatory processes of various severity. The concentration of procalcitonin was most likely to increase by 235.0% 3 days after alternating (every other day) administration of 0.02 ml of flosterone and 0.02 ml of S.aureus 108 No. 209. It was slightly lower by 120.0% under the same conditions experiment after 14 days. This indicator probably increased by 65% 14 days after the 7-time introduction of S.aureus 108 #209. In the rest of the experimental animals, the PCT concentration did not change. Conclusions. The determination of haptoglobin reflects, first of all, the primary activation of the inflammatory process, which was enhanced by the hormonal drug flosteron. However, its determination can be effective over a longer period of time, as several factors lead to a bacterial infection, reinforcing each other. At the same time, the synthesis of ceruloplasmin increases precisely during the first three days of the infectious process, which turns it into an effective marker for detecting early infectious complications. The dynamics of changes in the level of C-reactive protein in blood serum showed the highest correlation with the activity of the infectious process, which proves its high efficiency for detecting inflammatory processes of various severity, choosing adequate treatment and predicting the course of the disease.
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